Evolution of Bacillus thuringiensis Cry toxins insecticidal activity

A Bravo, I Gómez, H Porta… - Microbial …, 2013 - Wiley Online Library
Insecticidal Cry proteins produced by Bacillus thuringiensis are use worldwide in transgenic
crops for efficient pest control. Among the family of Cry toxins, the three domain Cry family is …

Bt toxin modification for enhanced efficacy

BR Deist, MA Rausch, MT Fernandez-Luna, MJ Adang… - Toxins, 2014 - mdpi.com
Insect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for
pest suppression. Here we review the different strategies that have been employed to …

Contained use of bacteriophages: risk assessment and biosafety recommendations

C Verheust, K Pauwels, J Mahillon, DR Helinski… - Applied …, 2010 - liebertpub.com
Bacteriophages are bacterial viruses and consist of a single-or double-stranded DNA or
RNA protected by a protein capsid. They are able to infect bacteria by injecting their nucleic …

Making 3D-cry toxin mutants: Much more than a tool of understanding toxins mechanism of action

S Vílchez - Toxins, 2020 - mdpi.com
3D-Cry toxins, produced by the entomopathogenic bacterium Bacillus thuringiensis, have
been extensively mutated in order to elucidate their elegant and complex mechanism of …

Molecular approaches towards development of novel Bacillus thuringiensis biopesticides

S Kaur - World Journal of Microbiology and Biotechnology, 2000 - Springer
Bacillus thuringiensis (Bt) has been used for control of lepidopteran, dipteran and
coleopteran insects for over three decades. Novel Bt strains harbouring new types of …

Molecular Approaches to Improve the Insecticidal Activity of Bacillus thuringiensis Cry Toxins

WA Lucena, PB Pelegrini, D Martins-de-Sa… - Toxins, 2014 - mdpi.com
Bacillus thuringiensis (Bt) is a gram-positive spore-forming soil bacterium that is distributed
worldwide. Originally recognized as a pathogen of the silkworm, several strains were found …

[HTML][HTML] Variant Cry1Ia toxins generated by DNA shuffling are active against sugarcane giant borer

KIC Craveiro, JEG Júnior, MCM Silva… - Journal of …, 2010 - Elsevier
Sugarcane giant borer (Telchin licus licus) is a serious sugarcane pest in Americas whose
endophytic lifestyle hampers effective chemical and biological controls. Therefore …

Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)

GR Oliveira, MCM Silva, WA Lucena, EYT Nakasu… - BMC …, 2011 - Springer
Background The cotton boll weevil (Anthonomus grandis) is a serious insect-pest in the
Americas, particularly in Brazil. The use of chemical or biological insect control is not …

A system for the directed evolution of the insecticidal protein from Bacillus thuringiensis

H Ishikawa, Y Hoshino, Y Motoki, T Kawahara… - Molecular …, 2007 - Springer
Theoretically, the activity of AB-type toxin molecules such as the insecticidal toxin (Cry toxin)
from B. thuringiensis, which have one active site and two binding site, is improved in parallel …

Molecular approaches for identification and construction of novel insecticidal genes for crop protection

S Kaur - World Journal of Microbiology and Biotechnology, 2006 - Springer
The insecticidal cry (crystal) genes from Bacillus thuringiensis (Bt) have been used for insect
control both as biopesticides and in transgenic plants. Discovery of new insecticidal genes is …