Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Obesity and diabetes now considered global epidemics. The prevalence rates of diabetes
are increasing in parallel with the rates of obesity and the strong connection between these …

The lineage relationships among the hundreds of cell types generated during development
are difficult to reconstruct. A recent method, GESTALT, used CRISPR–Cas9 barcode editing …

INTRODUCTION The developmental path by which a fertilized egg gives rise to the cells of a
multicellular organism is termed the cell lineage. In 1983, John Sulston and colleagues …

Implications Because of its fully sequenced genome, easy genetic manipulation, high
fecundity, external fertilization and rapid development, and nearly transparent embryo …

CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or
germline mutants of model organisms. Key constraints in vivo remain the expression and …

Genome-editing tools, and in particular those based on CRISPR–Cas (clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein) systems, are …

The CRISPR/Cas9 system is a powerful genome editing tool and has been widely used for
biomedical research. However, many challenges, such as off-target effects and lack of easy …

The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …

Genetic screens are powerful tools for the functional annotation of genomes. In the context of
multicellular organisms, interrogation of gene function is greatly facilitated by methods that …