Gene synthesis demystified
DNA fabrication of genetic cassettes at base-level precision is transforming genetic
engineering from a laborious art to an information-driven discipline. Although substantial …
engineering from a laborious art to an information-driven discipline. Although substantial …
Toward minimal bacterial cells: evolution vs. design
Recent technical and conceptual advances in the biological sciences opened the possibility
of the construction of newly designed cells. In this paper we review the state of the art of cell …
of the construction of newly designed cells. In this paper we review the state of the art of cell …
Deciphering the rules by which 5′-UTR sequences affect protein expression in yeast
The 5′-untranslated region (5′-UTR) of mRNAs contains elements that affect expression,
yet the rules by which these regions exert their effect are poorly understood. Here, we …
yet the rules by which these regions exert their effect are poorly understood. Here, we …
Manipulating nucleosome disfavoring sequences allows fine-tune regulation of gene expression in yeast
Understanding how precise control of gene expression is specified within regulatory DNA
sequences is a key challenge with far-reaching implications. Many studies have focused on …
sequences is a key challenge with far-reaching implications. Many studies have focused on …
Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
M Bujara, M Schümperli, R Pellaux… - Nature chemical …, 2011 - nature.com
Recruiting complex metabolic reaction networks for chemical synthesis has attracted
considerable attention but frequently requires optimization of network composition and …
considerable attention but frequently requires optimization of network composition and …
[HTML][HTML] A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates
Y Vazana, Y Barak, T Unger, Y Peleg… - Biotechnology for …, 2013 - Springer
Background Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …
Strategies for protein synthetic biology
R Grünberg, L Serrano - Nucleic acids research, 2010 - academic.oup.com
Proteins are the most versatile among the various biological building blocks and a mature
field of protein engineering has lead to many industrial and biomedical applications. But the …
field of protein engineering has lead to many industrial and biomedical applications. But the …
[HTML][HTML] End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis
Background Synthetic biology aims to engineer biological systems for desired behaviors.
The construction of these systems can be complex, often requiring genetic reprogramming …
The construction of these systems can be complex, often requiring genetic reprogramming …
Synthetic biology: tools to design, build, and optimize cellular processes
The general central dogma frames the emergent properties of life, which make biology both
necessary and difficult to engineer. In a process engineering paradigm, each biological …
necessary and difficult to engineer. In a process engineering paradigm, each biological …
Multiplex pairwise assembly of array-derived DNA oligonucleotides
While the cost of DNA sequencing has dropped by five orders of magnitude in the past
decade, DNA synthesis remains expensive for many applications. Although DNA …
decade, DNA synthesis remains expensive for many applications. Although DNA …