An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins

E Nji, Y Chatzikyriakidou, M Landreh… - Nature Communications, 2018 - nature.com
Membrane bilayers are made up of a myriad of different lipids that regulate the functional
activity, stability, and oligomerization of many membrane proteins. Despite their importance …

Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site

WY Wahlgren, E Dunevall, RA North, A Paz… - Nature …, 2018 - nature.com
Many pathogenic bacteria utilise sialic acids as an energy source or use them as an external
coating to evade immune detection. As such, bacteria that colonise sialylated environments …

Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters

M Coincon, P Uzdavinys, E Nji, DL Dotson… - Nature structural & …, 2016 - nature.com
To fully understand the transport mechanism of Na+/H+ exchangers, it is necessary to
clearly establish the global rearrangements required to facilitate ion translocation. Currently …

Crystal structure of the sodium–proton antiporter NhaA dimer and new mechanistic insights

C Lee, S Yashiro, DL Dotson, P Uzdavinys… - Journal of General …, 2014 - rupress.org
Sodium–proton antiporters rapidly exchange protons and sodium ions across the membrane
to regulate intracellular pH, cell volume, and sodium concentration. How ion binding and …

Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters

M Landreh, EG Marklund, P Uzdavinys… - Nature …, 2017 - nature.com
Na+/H+ antiporters are found in all kingdoms of life and exhibit catalysis rates that are
among the fastest of all known secondary-active transporters. Here we combine ion mobility …

A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins

F Angius, O Ilioaia, A Amrani, A Suisse, L Rosset… - Scientific reports, 2018 - nature.com
Membrane protein (MP) overproduction is one of the major bottlenecks in structural
genomics and biotechnology. Despite the emergence of eukaryotic expression systems …

Antimicrobial, anti-inflammatory and anti-arthritic activity of hemolymph lectin (NagLec) isolated from the freshwater crab, Oziotelphusa naga

F Vargila, SMM Bai, JVJ Mary, M Ramesh - Fish & Shellfish Immunology, 2023 - Elsevier
Lectins are non-immune glycoproteins or proteins having a unique capacity to interact with
carbohydrate ligands found on the surface of their host cells. In the present investigation, the …

General and modular strategy for designing potent, selective, and pharmacologically compliant inhibitors of rhomboid proteases

A Tichá, S Stanchev, KR Vinothkumar, DC Mikles… - Cell chemical …, 2017 - cell.com
Rhomboid-family intramembrane proteases regulate important biological processes and
have been associated with malaria, cancer, and Parkinson's disease. However, due to the …

Structure of cytosine transport protein CodB provides insight into nucleobase‐cation symporter 1 mechanism

CE Hatton, DH Brotherton, M Spencer… - The EMBO …, 2022 - embopress.org
CodB is a cytosine transporter from the Nucleobase‐Cation‐Symport‐1 (NCS1) transporter
family, a member of the widespread LeuT superfamily. Previous experiments with the …

Mechanism of substrate binding and transport in BASS transporters

P Becker, F Naughton, D Brotherton… - Elife, 2023 - elifesciences.org
The bile acid sodium symporter (BASS) family transports a wide array of molecules across
membranes, including bile acids in humans, and small metabolites in plants. These …