Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Versatility of cyclodextrins in self-assembly systems of amphiphiles

L Jiang, Y Yan, J Huang - Advances in Colloid and Interface Science, 2011 - Elsevier
Recently, cyclodextrins (CDs) were found to play important yet complicated (or even
apparently opposite sometimes) roles in self-assembly systems of amphiphiles or …

Rapid fabrication of precise high-throughput filters from membrane protein nanosheets

YM Tu, W Song, T Ren, Y Shen, R Chowdhury… - Nature Materials, 2020 - nature.com
Biological membranes are ideal for separations as they provide high permeability while
maintaining high solute selectivity due to the presence of specialized membrane protein …

Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents

M Dezi, A Di Cicco, P Bassereau… - Proceedings of the …, 2013 - National Acad Sciences
Giant unilamellar vesicles (GUVs) are convenient biomimetic systems of the same size as
cells that are increasingly used to quantitatively address biophysical and biochemical …

GraDeR: membrane protein complex preparation for single-particle cryo-EM

F Hauer, C Gerle, N Fischer, A Oshima… - Structure, 2015 - cell.com
We developed a method, named GraDeR, which substantially improves the preparation of
membrane protein complexes for structure determination by single-particle cryo-electron …

Structural analysis of CYP2R1 in complex with vitamin D3

N Strushkevich, SA Usanov, AN Plotnikov… - Journal of molecular …, 2008 - Elsevier
The activation of vitamin D to its hormonal form is mediated by cytochrome P450 enzymes.
CYP2R1 catalyzes the initial step converting vitamin D into 25-hydroxyvitamin D. A CYP2R1 …

Structural insights into phosphatidylethanolamine N-methyltransferase PmtA mediating bacterial phosphatidylcholine synthesis

SD Salsabila, J Kim - Science Advances, 2024 - science.org
Phosphatidylethanolamine N-methyltransferase (PmtA) catalyzes the biosynthesis of
phosphatidylcholine (PC) from phosphatidylethanolamine (PE). Although PC is one of the …

Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples

N Das, DT Murray, TA Cross - Nature protocols, 2013 - nature.com
Solid-state NMR spectroscopy has been used successfully for characterizing the structure
and dynamics of membrane proteins as well as their interactions with other proteins in lipid …

Proteomic analysis of the urothelial cancer landscape

FF Dressler, F Diedrichs, D Sabtan, S Hinrichs… - Nature …, 2024 - nature.com
Urothelial bladder cancer (UC) has a wide tumor biological spectrum with challenging
prognostic stratification and relevant therapy-associated morbidity. Most molecular …

Reconstitution of membrane proteins into polymer-supported membranes for probing diffusion and interactions by single molecule techniques

F Roder, S Waichman, D Paterok, R Schubert… - Analytical …, 2011 - ACS Publications
We have established a robust and versatile analytical platform for probing membrane
protein function in a defined lipid environment on solid supports. This approach is based on …