Systems biology and synthetic biology are increasingly used to examine and modulate
complex biological systems. As such, many issues arising during scaling-up microbial …

In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Background Accurate interpretation of quantitative PCR (qPCR) data requires normalization
using constitutively expressed reference genes. Ribosomal RNA is often used as a …

Bacteria have long been the favorite expression system for recombinant protein production.
However, the flaw of the system is that insoluble and inactive proteins are co-produced due …

Analytical approaches for a comprehensive understanding of the metabolic networks in
microbial cultures are mostly based on small-scale cultures which are in a steady state or …

Bacteria have evolved diverse mechanisms to survive environments with antibiotics.
Temperature is both a key factor that affects the survival of bacteria in the presence of …

The temperature inducible expression system, based on the pL and/or pR phage lambda
promoters regulated by the thermolabile cI857 repressor has been widely use to produce …

Large‐scale production and incorporation of titanium dioxide nanoparticles (NP‐TiO2) in
consumer products leads to their potential release into the environment and raises the …

ABSTRACT Ultrahigh-molecular-weight poly [(R)-3-hydroxybutyrate][UHMW-P (3HB)]
synthesized by genetically engineered Escherichia coli is an environmentally friendly …

During the past two decades, there has been an explosion of new knowledge and
techniques in the field of recombinant protein expression. However, over-expression of …