Computational analysis of microbial flow cytometry data

P Rubbens, R Props - MSystems, 2021 - Am Soc Microbiol
Flow cytometry is an important technology for the study of microbial communities. It grants
the ability to rapidly generate phenotypic single-cell data that are both quantitative …

Rapid detection of microbiota cell type diversity using machine-learned classification of flow cytometry data

BD Özel Duygan, N Hadadi, AF Babu… - Communications …, 2020 - nature.com
The study of complex microbial communities typically entails high-throughput sequencing
and downstream bioinformatics analyses. Here we expand and accelerate microbiota …

Coculturing bacteria leads to reduced phenotypic heterogeneities

J Heyse, B Buysschaert, R Props… - Applied and …, 2019 - Am Soc Microbiol
Isogenic bacterial populations are known to exhibit phenotypic heterogeneity at the single-
cell level. Because of difficulties in assessing the phenotypic heterogeneity of a single taxon …

PhenoGMM: Gaussian mixture modeling of cytometry data quantifies changes in microbial community structure

P Rubbens, R Props, FM Kerckhof, N Boon… - MSphere, 2021 - Am Soc Microbiol
Microbial flow cytometry can rapidly characterize the status of microbial communities. Upon
measurement, large amounts of quantitative single-cell data are generated, which need to …

Flow cytometry for on-line microbial regrowth monitoring in a membrane filtration plant: pilot-scale case study for wastewater reuse

T Pluym, C García-Timermans, S Vervloet… - … : Water Research & …, 2023 - pubs.rsc.org
The use of advanced membrane processes, such as reverse osmosis (RO) are of great
importance for process water and drinking water production. As the water quality is bound to …

Fast quantification of gut bacterial species in cocultures using flow cytometry and supervised classification

CC van de Velde, C Joseph, A Biclot… - ISME …, 2022 - academic.oup.com
A bottleneck for microbial community experiments with many samples and/or replicates is
the fast quantification of individual taxon abundances, which is commonly achieved through …

Predicting the presence and abundance of bacterial taxa in environmental communities through flow cytometric fingerprinting

J Heyse, F Schattenberg, P Rubbens, S Müller… - Msystems, 2021 - Am Soc Microbiol
Microbiome management research and applications rely on temporally resolved
measurements of community composition. Current technologies to assess community …

Cyt‐Geist: Current and future challenges in cytometry: Reports of the CYTO 2018 conference workshops

K Czechowska, J Lannigan, L Wang… - Cytometry Part …, 2019 - Wiley Online Library
Since the late 1960s, FCM has given the biologist the ability to interrogate both normal and
diseased biological conditions at the single-cell level in a statistically relevant way (1). For …

Unlocking the mechanism of action: a cost-effective flow cytometry approach for accelerating antimicrobial drug development

F Mermans, H De Baets… - Microbiology …, 2024 - Am Soc Microbiol
Antimicrobial resistance is one of the greatest challenges to global health. While the
development of new antimicrobials can combat resistance, low profitability reduces the …

Temporal influence of different antibiotics onto the inhibition of Escherichia coli bacterium grown in different media

IA Ratiu, VR Plugaru, P Pomastowski… - Analytical …, 2019 - Elsevier
Escherichia coli (E. coli) is a Gram-negative bacterium commonly found in the lower
intestine of warm-blooded organisms, including humans. Although the majority of the strains …