Intensiometric genetically encoded biosensors, based on allosteric modulation of the
fluorescence of a single fluorescent protein, are powerful tools for enabling imaging of …

Feynman commented that “Everything that living things do can be understood in terms of the
jiggling and wiggling of atoms”. Proteins can jiggle and wiggle large structural elements …

Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Abstract L-Ergothioneine (ET), the 2-thioimidazole derivative of trimethylhistidine, is
biosynthesized by select fungi and bacteria, notably Mycobacterium tuberculosis, and …

Over the past decades, single-molecule and super-resolution microscopy have advanced
and represent essential tools for life science research. There is, however, a growing gap …

(Micro) organisms are exposed to fluctuating environmental conditions, and adaptation to
stress is essential for survival. Increased osmolality (hypertonicity) causes outflow of water …

ATP-binding cassette (ABC) transporters use the energy of ATP hydrolysis to transport a
large diversity of molecules actively across biological membranes. A combination of …

Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC)
permeases serve as the primary import pathway for many micronutrients including the first …

Recent developments concerning large protein nanopores suggest a new approach to
structure profiling of native folded proteins. In this work, the large vestibule of Mycobacterium …