Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

Genetic code expansion allows unnatural (non-canonical) amino acid incorporation into
proteins of interest by repurposing the cellular translation machinery. The development of …

Genetically introducing novel chemical bonds into proteins provides innovative avenues for
biochemical research, protein engineering, and biotherapeutic applications. Recently, latent …

Drugs with a covalent mechanism of action benefit from enhanced potency, selectivity, and
in vivo efficacy. Historically, the only covalent drugs on the market have been covalent small …

Abstract Selenocysteine (Sec), the 21st genetically encoded amino acid, is structurally
similar to cysteine (Cys) but with a sulfur to selenium replacement. This small change …

The ability to construct a peptide or protein in a spatio-specific manner is of great interest for
therapeutic and biochemical research. However, the various functional groups present in …

Metabolic oligosaccharide engineering (MOE) is a classical chemical approach to perturb,
profile and perceive glycans in physiological systems, but probes upon bioorthogonal …

Protein identification has gone beyond simply using protein/peptide tags and labeling
canonical amino acids. Genetic code expansion has allowed residue-or site-specific …

The prenylation of peptides and proteins is an important post-translational modification
observed in vivo. We report that the Pd-catalyzed Tsuji–Trost allylation with a …

Peptides and proteins can be either synthesized using solid-phase peptide synthesis
(SPPS) or by applying a combination of SPPS and ligation approaches to address …