Abstract The Japanese medaka, Oryzias latipes, is a vertebrate teleost model with a long
history of genetic research. A number of unique features and established resources …

Stem cells have the potential for self-renewal and differentiation. First stem cell cultures were
derived 30 years ago from early developing mouse embryos. These are pluripotent …

Gene targeting (GT) can introduce subtle alterations into a particular locus and represents a
powerful tool for genome editing. Engineered zinc finger nucleases (ZFNs) are effective for …

In many species of aquaculture importance, all-female and sterile populations possess
superior productivity due to faster growth and a relatively homogenous size of individuals …

Mechanisms of the animal reproductive development are an important research field in life
sciences. The study of the reproductive development and regulatory mechanisms in fishes is …

Germ cells of diverse animal species have a unique membrane-less organelle called germ
plasm (GP). GP is usually associated with mitochondria and contains RNA binding proteins …

Transgenesis is the revolutionary science of making a genetically modified organism.
Interestingly, fish offer easier genetic manipulation compared to mammalian counterpart, as …

Transgenic (Tg) animal technology is one of the growing areas in biology. Various Tg
technologies, each with its own advantages and disadvantages, are available for generating …

Zinc finger nucleases (ZFNs) can generate targeted gene disruption (GD) directly in
developing embryos of zebrafish, mouse and human. In the fish medaka, ZFNs have been …

Transcription activator-like effector nucleases (TALENs) are used for gene knockout and
genome-editing studies in zebrafish, and these techniques have the potential to be applied …