Helicases are ubiquitous motors involved in almost all aspects of nucleic acid metabolism;
therefore, revealing their unwinding behaviors and mechanisms is fundamentally and …

Helicases are molecular motors that translocate along single-stranded DNA and unwind
duplex DNA. They rely on the consumption of chemical energy from nucleotide hydrolysis to …

The structural diversity of biological macromolecules in different environments contributes
complexity to enzymological processes vital for cellular functions. Fluorescence resonance …

Single-molecule surface-induced fluorescence attenuation (smSIFA) is a precise method for
studying the vertical movement of biological macromolecules using two-dimensional …

Flexible regions in biomolecular complexes, although crucial to understanding structure–
function relationships, are often unclear in high-resolution crystal structures. In this study, we …

Physical biology is an interdisciplinary field that bridges biology with physical sciences and
engineering. Single-molecule physical biology focuses on dynamics of individual …

Many years of fundamental studies on viral genome packaging motors have led to fruitful
applications. The double-stranded DNA (dsDNA) viruses package their genomes into …

The RecQ family of helicases are important for maintenance of genomic integrity. Although
functions of constructive subdomains of this family of helicases have been extensively …

DNA 聚合酶是执行DNA 复制和修复的重要蛋白, 由于其只能从5′ 向3′ 方向聚合,
所以在聚合双链DNA 时会有两种模式: 其一是先打开DNA 双链, 让其暴露出3′-5 …

The reversible generation and capture of certain electrophilic quinone methide
intermediates support dynamic reactions with DNA that allow for migration and transfer of …