3000 new references added since the first editionGives information necessary to produce
embryos totally through in vitro techniques Shows commercial applications of embryo and …

Improvements on embryo micromanipulation techniques led to the use of embryo bisection
technology in commercial embryo transfer programs, and made possible the direct genetic …

My research awarded includes contributions to cryopreservation and sexing of bovine
embryos produced in vitro and in vivo, as follows;(1) In vivo-derived morulae and blastocysts …

To make bovine embryo sexing under farm conditions more feasible we developed a
simplified protocol utilizing manual biopsy and detection of the Y chromosome directly from …

The objective of this study was to establish a rapid and reliable PCR method for the sexing
of 8-to 16-cell stage bovine embryos. The BOV97M and bovine 1.715 satellite DNA …

We described a procedure for multiple genotype analysis (determination of sex and of three
genetic markers) from a single cell derived from bovine preimplantation embryo. It consists …

Bovine embryos cultured in serum-containing media abnormally accumulate lipids in the
cytoplasm. This is well known to contribute to their higher susceptibility to cryopreservation …

Sex determination of livestock is performed to achieve the objectives of livestock breeding
programmes. Techniques for sex determination have evolved from karyotyping to detecting …

The cellular composition and viability of intact, IVP embryos were compared with those of
demi-and quarter-embryos produced by bisection of IVP morulae and blastocysts. Embryos …

为研究藏羚羊初生性别比例, 判断母羊怀孕期间有无偏性选择孕育过程, 根据藏羚羊繁殖特点,
在可可西里自然保护区卓乃湖藏羚羊产羔地收集到116 份藏羚羊胎盘样品 …