Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
[HTML][HTML] Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing
Bacterial toxins represent a vast reservoir of biochemical diversity that can be repurposed for
biomedical applications. Such proteins include a group of predicted interbacterial toxins of …
biomedical applications. Such proteins include a group of predicted interbacterial toxins of …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
Genetically modified crops: current status and future prospects
K Kumar, G Gambhir, A Dass, AK Tripathi, A Singh… - Planta, 2020 - Springer
Main conclusion While transgenic technology has heralded a new era in crop improvement,
several concerns have precluded their widespread acceptance. Alternative technologies …
several concerns have precluded their widespread acceptance. Alternative technologies …
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
CRISPR–Cas9 is poised to become the gene editing tool of choice in clinical contexts. Thus
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
[HTML][HTML] Multiplexed CRISPR technologies for gene editing and transcriptional regulation
NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …
expressed at once, have facilitated powerful biological engineering applications, vastly …
[HTML][HTML] The CRISPR tool kit for genome editing and beyond
M Adli - Nature communications, 2018 - nature.com
CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …
[HTML][HTML] Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants
To meet increasing global food demand, breeders and scientists aim to improve the yield
and quality of major food crops. Plant diseases threaten food security and are expected to …
and quality of major food crops. Plant diseases threaten food security and are expected to …
[HTML][HTML] CRISPR for crop improvement: an update review
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …
approaches has opened up possibilities to breed for almost any given desirable trait …