Metabolic engineering has allowed the production of a diverse number of valuable
chemicals using microbial organisms. Many biological challenges for improving bio …

Transcription factor (TF)-based biosensors are widely used for the detection of metabolites
and the regulation of cellular pathways in response to metabolites. Several challenges …

Genome-scale metabolic networks (GSMs) are fundamental systems biology
representations of a cell's entire set of stoichiometrically balanced reactions. However, such …

Metabolic addiction, an organism that is metabolically addicted with a compound to maintain
its growth fitness, is an underexplored area in metabolic engineering. Microbes with heavily …

Metabolite biosensors based on metabolite-responsive transcription factors are key
synthetic biology components for sensing and precisely controlling cellular metabolism …

Recent advances in synthetic biology have enabled the construction of molecular circuits
that operate across multiple scales of cellular organization, such as gene regulation …

Escherichia coli FadR is a transcription factor regulated by acyl‐CoA thioester binding that
optimizes fatty acid (FA) metabolism in response to environmental FAs. FadR represses the …

Bacteria can be harnessed to synthesise high-value chemicals. A promising strategy for
increasing productivity uses inducible control systems to switch metabolism from growth to …

Highlights•Feedback control can mitigate the impact of the ribosomal cost.•Feedback control
alleviates metabolic burden caused by engineered metabolic pathways.•Metabolic switch …

Metabolism plays a central role in cell physiology because it provides the molecular
machinery for growth. At the genome-scale, metabolism is made up of thousands of …