▪ Abstract In just a few short years, the chemical ligation of unprotected peptide segments in
aqueous solution has established itself as the most practical method for the total synthesis of …

Although chemists can synthesize virtually any small organic molecule, our ability to
rationally manipulate the structures of proteins is quite limited, despite their involvement in …

A protein semisynthesis method—expressed protein ligation—is described that involves the
chemoselective addition of a peptide to a recombinant protein. This method was used to …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

A major goal in structural biology is to unravel how molecular machines function in detail. To
that end, solution-state NMR spectroscopy is ideally suited as it is able to study biological …

The matrix metalloproteinases (MMP)-2 and-9, also known as the gelatinases have been
long recognized as major contributors to the proteolytic degradation of extracellular matrix …

Bioorthogonal chemistry involves selective biocompatible reactions between functional
groups that are not normally present in biology. It has been used to probe biomolecules in …

1. INTRODUCTION In eukaryotic cells, inheritable information is stored in a nucleoprotein
complex referred to as chromatin. 1 This genome architecture serves two key purposes. On …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

▪ Abstract Proteins have become accessible targets for chemical synthesis. The basic
strategy is to use native chemical ligation, Staudinger ligation, or other orthogonal chemical …