Highlights•Formate dehydrogenases (FDHs) can catalyze reduction of CO 2 to HCOO−.•
Immobilization can improve FDH kinetics for CO 2 reduction.•Non-natural electron donors …

Directed enzyme evolution has revolutionized the rapid development of enzymes with
desired properties. However, the lack of a high-throughput method to identify the most …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Fast screening of enzyme variants is crucial for tailoring biocatalysts for the asymmetric
synthesis of non-natural chiral chemicals, such as amines. However, most existing …

Formate can be envisioned at the core of a carbon-neutral bioeconomy, where it is produced
from CO2 by (electro-) chemical means and converted into value-added products by …

This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Synthetic biology has brought about a conceptual shift in our ability to redesign microbial
metabolic networks. Combining metabolic pathway-modularization with growth-coupled …

Nitrogenases are the only known enzymes that reduce molecular nitrogen (N2) to ammonia.
Recent findings have demonstrated that nitrogenases also reduce the greenhouse gas …

Noncanonical redox cofactors are attractive low-cost alternatives to nicotinamide adenine
dinucleotide (phosphate)(NAD (P)+) in biotransformation. However, engineering enzymes to …

Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …