Cryoprotectant toxicity: facts, issues, and questions
BP Best - Rejuvenation research, 2015 - liebertpub.com
High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during
cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become …
cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become …
Strategies for stabilization of enzymes in organic solvents
V Stepankova, S Bidmanova, T Koudelakova… - Acs …, 2013 - ACS Publications
One of the major barriers to the use of enzymes in industrial biotechnology is their
insufficient stability under processing conditions. The use of organic solvent systems instead …
insufficient stability under processing conditions. The use of organic solvent systems instead …
A new method to increase selectivity of transglutaminase mediated PEGylation of salmon calcitonin and human growth hormone
A Mero, M Schiavon, FM Veronese, G Pasut - Journal of controlled release, 2011 - Elsevier
Modification of therapeutic proteins and peptides by polyethylene glycol (PEG) conjugation
is a well-known approach to improve the pharmacological properties of drugs. Several …
is a well-known approach to improve the pharmacological properties of drugs. Several …
Immobilized crosslinked pectinase preparation on porous ZSM-5 zeolites as reusable biocatalysts for ultra-efficient hydrolysis of β-glycosidic bonds
In this study, we immobilized pectinase preparation on porous zeolite ZSM-5 as an enzyme
carrier. We realized this immobilized enzyme catalyst, pectinase preparation@ ZSM-5, via a …
carrier. We realized this immobilized enzyme catalyst, pectinase preparation@ ZSM-5, via a …
Effects of organic solvents on the activity of free and immobilised laccase from Rhus vernicifera
YY Wan, R Lu, L Xiao, YM Du, T Miyakoshi… - International journal of …, 2010 - Elsevier
Rhus laccase (RL) was covalently immobilised onto chitosan, and the effects of
immobilisation on pH optimum, enzyme activity, thermostability, and re-use evaluated, using …
immobilisation on pH optimum, enzyme activity, thermostability, and re-use evaluated, using …
Organic co‐solvents affect activity, stability and enantioselectivity of haloalkane dehalogenases
V Stepankova, J Damborsky… - Biotechnology …, 2013 - Wiley Online Library
Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological
applications, including biocatalysis. The use of organic co‐solvents to solubilize their …
applications, including biocatalysis. The use of organic co‐solvents to solubilize their …
The relationship between the cross-linker on chitosan-coated magnetic nanoparticles and the properties of immobilized papain
S Mostaraddi, M Pazhang, M Ebadi-Nahari… - Molecular …, 2023 - Springer
The immobilized enzymes' properties can be affected by cross-linkers on the surface of
supports. To study how cross-linkers alter enzymes function, chitosan-coated magnetic …
supports. To study how cross-linkers alter enzymes function, chitosan-coated magnetic …
Effect of sorbitol and glycerol on the stability of trypsin and difference between their stabilization effects in the various solvents
M Pazhang, F Mehrnejad, Y Pazhang… - Biotechnology and …, 2016 - Wiley Online Library
The effect of glycerol and sorbitol on the stability of porcine pancreas trypsin was
investigated in this work. Molecular dynamics simulation and thermostability results showed …
investigated in this work. Molecular dynamics simulation and thermostability results showed …
Immobilization of papain on nitrogen-doped graphene quantum dots improves the enzymatic properties and makes it a biosensor for cystatin C
F Mirzaei, H Valizadeh, M Pazhang - Process Biochemistry, 2022 - Elsevier
In recent years enzymes had many applications, such as catalysts in organic synthesis and
producing biosensors. In this study, nitrogen-doped graphene quantum dots (NGQDs) were …
producing biosensors. In this study, nitrogen-doped graphene quantum dots (NGQDs) were …
A novel approach to prepare insulin-loaded poly (lactic-co-glycolic acid) microcapsules and the protein stability study
The purpose of this study was to propose a new preparation method to fabricate insulin-
loaded poly (lactic-coglycolic acid)(PLGA) microparticles satisfying protein loading, release …
loaded poly (lactic-coglycolic acid)(PLGA) microparticles satisfying protein loading, release …