The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing
method. This method has been used in a variety of organisms and cell types over the past …

Organism-level systems biology aims to identify, analyze, control and design cellular circuits
in organisms. Many experimental and computational approaches have been developed …

Tissue environment plays a powerful role in establishing and maintaining the distinct
phenotypes of resident macrophages, but the underlying molecular mechanisms remain …

Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and …

Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease
Cas9 has become a popular method for genome editing in several organisms. Most HDR …

CRISPR/Cas9-based genome editing can easily generate knockout mouse models by
disrupting the gene sequence, but its efficiency for creating models that require either …

Neutrophil granule serine proteases contribute to immune responses through cleavage of
microbial toxins and structural proteins. They induce tissue damage and modulate …

Synaptic pruning is a fundamental process of neuronal circuit refinement in learning and
memory. Accumulating evidence suggests that glia participates in sculpting the neuronal …

Key message Site-directed mutagenesis of nitrate reductase genes using direct delivery of
purified Cas9 protein preassembled with guide RNA produces mutations efficiently in …

The CRISPR/Cas9 system is a powerful tool for elucidating the roles of genes in a wide
variety of organisms including mice. To obtain genetically modified embryos or mice by this …