The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing
method. This method has been used in a variety of organisms and cell types over the past …

Metformin, the most prescribed antidiabetic medicine, has shown other benefits such as anti-
ageing and anticancer effects,,–. For clinical doses of metformin, AMP-activated protein …

CRISPR-based approaches have quickly become a favored method to perturb genes to
uncover their functions. Here, we review the key considerations in the design of genome …

Recent work suggests that cholesterol metabolism impacts innate immune responses
against infection. However, the key enzymes or the natural products and mechanisms …

NOD-like receptors (NLRs) are pattern recognition receptors for diverse innate immune
responses. Self-oligomerization after engagement with a ligand is a generally accepted …

DNA cytosine methylation plays a vital role in repressing retrotransposons, and such
derepression is linked with developmental failure, tumorigenesis and aging. DNA …

CRISPR-mediated base editing can introduce single-nucleotide changes in the DNA of
living cells. One intriguing application of base editing is to screen pivotal amino acids for …

BACKGROUND With the recent development of CRISPR (clustered regularly interspaced
short palindromic repeats)/Cas9 genome editing technology, the possibility to genetically …

One of the fundamental discoveries in the field of biology is the ability to modulate the
genome and to monitor the functional outputs derived from genomic alterations. In order to …

During embryo development, DNA methylation is established by DNMT3A/3B and
subsequently maintained by DNMT1. While much research has been done in this field, the …