Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

EJ Thak, SJ Yoo, HY Moon, HA Kang - FEMS yeast research, 2020 - academic.oup.com
Yeasts are prominent hosts for the production of recombinant proteins from industrial
enzymes to therapeutic proteins. Particularly, the similarity of protein secretion pathways …

[PDF][PDF] CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae

Y Wang, X Li, X Chen, V Siewers - FEMS Yeast Research, 2022 - academic.oup.com
The rapid expansion of the application of pharmaceutical proteins and industrial enzymes
requires robust microbial workhorses for high protein production. The budding yeast …

Characterization of Feruloyl Esterase from Bacillus pumilus SK52.001 and Its Application in Ferulic Acid Production from De-Starched Wheat Bran

X Duan, Y Dai, T Zhang - Foods, 2021 - mdpi.com
Feruloyl esterase (FAE; EC 3.1. 1.73) catalyzes the hydrolysis of the 4-hydroxy-3-
methoxycinnamoyl group in an esterified sugar to assist in waste biomass degradation or to …

High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway

T Tian, X Wu, P Wu, X Lu, Q Wang, Y Lin… - … in Bioengineering and …, 2024 - frontiersin.org
Soy leghemoglobin, when bound to heme, imparts a meat-like color and flavor and can
serve as a substitute for animal-derived proteins. Enhancing cellular heme synthesis …

Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of …

T Shi, J Zhou, A Xue, H Lu, Y He, Y Yu - Biotechnology for Biofuels, 2021 - Springer
Background Kluyveromyces marxianus is a promising cell factory for producing bioethanol
and that raised a demand for a high yield of heterologous proteins in this species …

Coupling thermotolerance and high production of recombinant protein by CYR1N1546K mutation via cAMP signaling cascades

H Ren, Q Lan, S Zhou, Y Lyu, Y Yu, J Zhou… - Communications …, 2024 - nature.com
In recombinant protein-producing yeast strains, cells experience high production-related
stresses similar to high temperatures. It is possible to increase recombinant protein …

Efficient and markerless gene integration with SlugCas9-HF in Kluyveromyces marxianus

H Zhou, T Tian, J Liu, H Lu, Y Yu, Y Wang - Communications Biology, 2024 - nature.com
The nonconventional yeast Kluyveromyces marxianus has potential for industrial production,
but the lack of advanced synthetic biology tools for precise engineering hinders its rapid …

A Single Vaccination of IBDV Subviral Particles Generated by Kluyveromyces marxianus Efficiently Protects Chickens against Novel Variant and Classical IBDV …

D Yang, L Zhang, J Duan, Q Huang, Y Yu, J Zhou, H Lu - Vaccines, 2021 - mdpi.com
Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), is a
highly contagious and immunosuppressive disease in chickens worldwide. The novel …

Downregulation of ammonium uptake improves the growth and tolerance of Kluyveromyces marxianus at high temperature

Y Ai, T Luo, Y Yu, J Zhou, H Lu - MicrobiologyOpen, 2022 - Wiley Online Library
The growth and tolerance of Kluyveromyces marxianus at high temperatures decreased
significantly in the synthetic medium (SM), which is commonly used in industrial …

[HTML][HTML] High-Yield Expressed Human Ferritin Heavy-Chain Nanoparticles in K. marxianus for Functional Food Development

X Lu, L Liu, H Zhang, H Lu, T Tian, B Du, P Li, Y Yu… - Foods, 2024 - mdpi.com
The use of Generally Recognized as Safe (GRAS)-grade microbial cell factories to produce
recombinant protein-based nutritional products is a promising trend in developing food and …