In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

Analyzing proteins from single cells by tandem mass spectrometry (MS) has recently
become technically feasible. While such analysis has the potential to accurately quantify …

Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-
state heterogeneity and distinct cellular signaling patterns that remain obscured with …

Background Many biological processes, such as cell division cycle and drug resistance, are
reflected in protein covariation across single cells. This covariation can be quantified and …

Multiplexed and label-free mass spectrometry–based approaches with single-cell resolution
have attributed surprising heterogeneity to presumed homogenous cell populations. Even …

The field of single-cell omics is rapidly progressing. Although DNA and RNA sequencing-
based methods have dominated the field to date, global proteome profiling has also entered …

Sample preparation for single-cell proteomics is generally performed in a one-pot workflow
with multiple dispensing and incubation steps. These hours-long processes can be labor …

Single-cell proteomics (scProteomics) promises to advance our understanding of cell
functions within complex biological systems. However, a major challenge of current methods …

Single-cell methodologies and technologies have started a revolution in biology which until
recently has primarily been limited to deep sequencing and imaging modalities. With the …

Single-cell proteomics (SCP) has emerged as a powerful tool for detecting cellular
heterogeneity, offering unprecedented insights into biological mechanisms that are masked …