Transformation-associated recombination (TAR) cloning represents a unique tool for
isolation and manipulation of large DNA molecules. The technique exploits a high level of …

Plant metabolism represents an enormous repository of compounds that are of
pharmaceutical and biotechnological importance. Engineering plant metabolism into …

Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value
chemicals. However, compared with model genetic organisms such as Escherichia coli and …

DNA digital storage provides an alternative for information storage with high density and
long-term stability. Here, we report the de novo design and synthesis of an artificial …

The selective regulation of bacteria in complex microbial populations is key to controlling
pathogenic bacteria. CRISPR nucleases can be programmed to kill bacteria, but require an …

With the completion of the genome sequence, and development of an efficient conjugation-
based transformation system allowing the introduction of stable episomes, Phaeodactylum …

Understanding how to program biological functions into artificial DNA sequences remains a
key challenge in synthetic genomics. Here, we report the chemical synthesis and testing of …

With the advent of the genomics era, heterologous gene expression has been used
extensively as a means of accessing natural products (NPs) from environmental DNA …

The worldwide COVID-19 pandemic caused by the SARS-CoV-2 betacoronavirus has
highlighted the need for a synthetic biology approach to create reliable and scalable …

Current DNA assembly methods for preparing highly purified linear subassemblies require
complex and time-consuming in vitro manipulations that hinder their ability to construct …