The elicitation of anti-drug antibodies (ADA) against biotherapeutics can have detrimental
effects on drug safety, efficacy, and pharmacokinetics. The immunogenicity of …

A common limitation of recombinant protein production in bacteria is the formation of
insoluble protein aggregates known as inclusion bodies. The propensity of a given protein to …

Background Many enzymes of industrial interest are not in the market since they are bio-
produced as bacterial inclusion bodies, believed to be biologically inert aggregates of …

Protein aggregation is a major bottleneck during the bacterial production of recombinant
proteins. In general, the induction of gene expression at sub‐optimal growth temperatures …

Background The overproduction of recombinant proteins in host cells often leads to their
misfolding and aggregation. Previous attempts to increase the solubility of recombinant …

Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Since the dawn of time, or at least the dawn of recombinant DNA technology (which for many
of today's scientists is the same thing), investigators have been cloning and expressing …

Increasing evidence indicates that protein aggregation in bacteria does not necessarily
imply loss of biological activity. Here, we have investigated the effect of growth-temperature …

When massively expressed in bacteria, recombinant proteins often tend to misfold and
accumulate as soluble and insoluble nonfunctional aggregates. A general strategy to …

Background The solubility of recombinant proteins expressed in bacteria is often
disappointingly low. Several strategies have been developed to improve the yield and one …