Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions

P Picotti, R Aebersold - Nature methods, 2012 - nature.com
Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …

Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present

M Bantscheff, S Lemeer, MM Savitski… - Analytical and bioanalytical …, 2012 - Springer
Mass-spectrometry-based proteomics is continuing to make major contributions to the
discovery of fundamental biological processes and, more recently, has also developed into …

Selected reaction monitoring applied to proteomics

S Gallien, E Duriez, B Domon - Journal of Mass Spectrometry, 2011 - Wiley Online Library
Selected reaction monitoring (SRM) performed on triple quadrupole mass spectrometers
has been the reference quantitative technique to analyze small molecules for several …

Bioanalytical LC–MS/MS of protein-based biopharmaceuticals

I van den Broek, WMA Niessen… - … of Chromatography B, 2013 - Elsevier
Biotechnology increasingly delivers highly promising protein-based biopharmaceutical
candidates to the drug development funnel. For successful biopharmaceutical drug …

Advancing the sensitivity of selected reaction monitoring‐based targeted quantitative proteomics

T Shi, D Su, T Liu, K Tang, DG Camp, WJ Qian… - …, 2012 - Wiley Online Library
Selected reaction monitoring (SRM)–also known as multiple reaction monitoring (MRM)–has
emerged as a promising high‐throughput targeted protein quantification technology for …

Targeted liquid chromatography‐tandem mass spectrometry analysis of proteins: Basic principles, applications, and perspectives

A Kulyyassov, M Fresnais, R Longuespée - Proteomics, 2021 - Wiley Online Library
Liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) is now the main
analytical method for the identification and quantification of peptides and proteins in …

Mass spectrometry‐based targeted quantitative proteomics: achieving sensitive and reproducible detection of proteins

ES Boja, H Rodriguez - Proteomics, 2012 - Wiley Online Library
Traditional shotgun proteomics used to detect a mixture of hundreds to thousands of
proteins through mass spectrometric analysis, has been the standard approach in research …

Development of a quantitative proteomics approach for cyclooxygenases and lipoxygenases in parallel to quantitative oxylipin analysis allowing the comprehensive …

NM Hartung, M Mainka, R Pfaff, M Kuhn… - Analytical and …, 2023 - Springer
Oxylipins derived from the cyclooxygenase (COX) and lipoxygenase (LOX) pathways of the
arachidonic acid (ARA) cascade are essential for the regulation of the inflammatory …

Recent advances in mass spectrometry: data independent analysis and hyper reaction monitoring

KP Law, YP Lim - Expert review of proteomics, 2013 - Taylor & Francis
New mass spectrometry (MS) methods, collectively known as data independent analysis
and hyper reaction monitoring, have recently emerged. These methods hold promises to …