Cell-free gene expression: methods and applications

AC Hunt, BJ Rasor, K Seki, HM Ekas, KF Warfel… - Chemical …, 2024 - ACS Publications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Achieving broad availability of SARS-CoV-2 detections via smartphone-based analysis

D Li, C Sun, X Mei, L Yang - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
With the development of COVID-19, widely available tests are in great demand. Naked-eye
SARS-CoV-2 test kits have recently been developed as home tests, but their sensitivity and …

A sensitive and specific fluorescent RT-LAMP assay for SARS-CoV-2 detection in clinical samples

KH Ooi, MM Liu, JR Moo, P Nimsamer… - ACS Synthetic …, 2022 - ACS Publications
The raging COVID-19 pandemic has created an unprecedented demand for frequent and
widespread testing to limit viral transmission. Reverse transcription loop-mediated …

Making the next generation of therapeutics: mRNA meets synthetic biology

A Hınçer, RE Ahan, E Aras, UOS Şeker - ACS synthetic biology, 2023 - ACS Publications
The development of mRNA-based therapeutics centers around the natural functioning of
mRNA molecules to provide the genetic information required for protein translation. To …

Double loop-stem hairpins mediated hybridization chain reaction: A multifunctional DNA molecular tool to produce the intact aptamer for label-free biosensing

X Peng, J Yang, W Liang, Y Sun, X Mei, G Zhang… - Sensors and Actuators B …, 2022 - Elsevier
A DNA molecular tool based on the double loop-stem (DL) hairpins mediated hybridization
chain reaction (D-HCR) system was proposed to produce the intact G-quadruplex (InG4) …

Synthetic biology enables field-deployable biosensors for water contaminants

B Saltepe, L Wang, B Wang - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Wastewater surveillance is a powerful tool to understand community profiling in terms of
health monitoring. Tracking biomarkers such as inorganic and organic pollutants, drugs, and …

Combating infectious diseases with synthetic biology

A Khan, J Ostaku, E Aras… - ACS Synthetic Biology, 2022 - ACS Publications
Over the past decades, there have been numerous outbreaks, including parasitic, fungal,
bacterial, and viral infections, worldwide. The rate at which infectious diseases are emerging …

[HTML][HTML] Gene circuit-based sensors

X Guo, M Li, X Zuo - Fundamental Research, 2024 - Elsevier
As an important branch of biosensors, gene circuit-based biosensors constructed based on
synthetic biology concepts can sense specific substances to be measured and converted …

Rapid enzymatic assays for fecal contamination in aquatic environment: challenges, advances and prospects

X Yuan, A Glidle, Z Yang, B Wang - TrAC Trends in Analytical Chemistry, 2024 - Elsevier
Routine monitoring of sanitation and hygiene to identify fecal contamination in aquatic
environments is an effective means to prevent threatening disease transmission. Compared …

RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics

IC Koksaldi, D Park, A Atilla, H Kang, J Kim… - ACS Synthetic …, 2024 - ACS Publications
In the era of the COVID-19 pandemic, the significance of point-of-care (POC) diagnostic
tools has become increasingly vital, driven by the need for quick and precise virus …