DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Background The analyses of amplification and melting curves have been shown to provide
valuable information on the quality of the individual reactions in quantitative PCR (qPCR) …

Digital polymerase chain reaction (dPCR) technology has provided a new technique for
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …

Abstract Background Quantitative PCR (qPCR) aims to measure the DNA or RNA
concentration in diagnostic and biological samples based on the quantification cycle (Cq) …

In the analysis of quantitative PCR (qPCR) data, the quantification cycle (Cq) indicates the
position of the amplification curve with respect to the cycle axis. Because Cq is directly …

Real-time quantitative polymerase-chain-reaction (qPCR) is a standard technique in most
laboratories used for various applications in basic research. Analysis of qPCR data is a …

OBJECTIVE Both glucose and triglyceride production are increased in type 2 diabetes and
nonalcoholic fatty liver disease (NAFLD). For decades, the leading hypothesis to explain …

Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-
qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if …

The particulate matter present in air pollution is a complex mixture of solid and liquid
particles that vary in size, origin, and composition, among which are polycyclic aromatic …