Advances in nucleic acid-based diagnostics of bacterial infections

KB Barken, JAJ Haagensen, T Tolker-Nielsen - Clinica Chimica Acta, 2007 - Elsevier
Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens
have evolved significantly over the last decade. Many of the new procedures are nucleic …

Developing nucleic acid-based electrical detection systems

M Gabig-Ciminska - Microbial cell factories, 2006 - Springer
Abstract Development of nucleic acid-based detection systems is the main focus of many
research groups and high technology companies. The enormous work done in this field is …

Automated detection and quantitation of bacterial RNA by using electrical microarrays

B Elsholz, R Wörl, L Blohm, J Albers, H Feucht… - Analytical …, 2006 - ACS Publications
Low-density electrical 16S rRNA specific oligonucleotide microarrays and an automated
analysis system have been developed for the identification and quantitation of pathogens …

Making target sites in large structured RNAs accessible to RNA-cleaving DNAzymes through hybridization with synthetic DNA oligonucleotides

C Nurmi, J Gu, A Mathai, JD Brennan… - Nucleic Acids …, 2024 - academic.oup.com
The 10–23 DNAzyme is one of the most active DNA-based enzymes, and in theory, can be
designed to target any purine-pyrimidine junction within an RNA sequence for cleavage …

Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction

D Resina, M Bollók, NK Khatri, F Valero… - Microbial Cell …, 2007 - Springer
Background The analysis of transcriptional levels of the genes involved in protein synthesis
and secretion is a key factor to understand the host organism's responses to recombinant …

Sandwich Hybridization Assay for Sensitive Detection of Dynamic Changes in mRNA Transcript Levels in Crude Escherichia coli Cell Extracts in Response to Copper …

D Thieme, P Neubauer, DH Nies… - Applied and …, 2008 - Am Soc Microbiol
Transcript quantification techniques usually rely on purified mRNAs. We report here a
solution-based sandwich hybridization assay for the quantification of mRNAs from …

Sandwich hybridization assay for in situ real-time cyanobacterial detection and monitoring: A review

P Gong, AK Antrim, SR Bickman, EG Cooley… - Biosensors, 2022 - mdpi.com
As cyanobacterial harmful algal bloom (cHAB) events increase in scale, severity, frequency,
and duration around the world, rapid and accurate monitoring and characterization tools …

Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli

T Heo, H Kang, S Choi, J Kim - Life, 2021 - mdpi.com
Synthetic biologists have applied biomolecular engineering approaches toward the goal of
novel biological devices and have shown progress in diverse areas of medicine and …

Application of an electric DNA‐chip for the expression analysis of bioprocess‐relevant marker genes of Bacillus subtilis

B Jürgen, KB Barken, S Tobisch… - Biotechnology and …, 2005 - Wiley Online Library
The knowledge of critical process‐relevant genes can be used for an improved control of
bioprocesses. So far bioprocess‐relevant marker genes can be analyzed by established …

Antisense RNA based down-regulation of RNaseE in E.coli

C Kemmer, P Neubauer - Microbial Cell Factories, 2006 - Springer
Background Messenger RNA decay is an important mechanism for controlling gene
expression in all organisms. The rate of the mRNA degradation directly affects the steady …