Lipopolysaccharide and the gut microbiota: considering structural variation

AE Mohr, M Crawford, P Jasbi, S Fessler… - FEBS …, 2022 - Wiley Online Library
Systemic inflammation is associated with chronic disease and is purported to be a main
pathogenic mechanism underlying metabolic conditions. Microbes harbored in the host …

Photodissociation mass spectrometry: new tools for characterization of biological molecules

JS Brodbelt - Chemical Society Reviews, 2014 - pubs.rsc.org
Photodissociation mass spectrometry combines the ability to activate and fragment ions
using photons with the sensitive detection of the resulting product ions by mass …

[HTML][HTML] Lipopolysaccharide structure and the phenomenon of low endotoxin recovery

A Gorman, AP Golovanov - European Journal of Pharmaceutics and …, 2022 - Elsevier
Lipopolysaccharide (LPS) is a cell-wall component of Gram-negative bacteria which
contributes to bacterial toxicity. During processes such as cell division, shedding of outer …

Shotgun analysis of rough-type lipopolysaccharides using ultraviolet photodissociation mass spectrometry

DR Klein, DD Holden, JS Brodbelt - Analytical chemistry, 2016 - ACS Publications
Detailed structural characterization of intact rough-type lipopolysaccharides (R-LPS) was
accomplished using a multi-stage mass spectrometry (MS3) strategy consisting of collision …

Mass spectrometry methodology in lipid analysis

L Li, J Han, Z Wang, J Liu, J Wei, S Xiong… - International journal of …, 2014 - mdpi.com
Lipidomics is an emerging field, where the structures, functions and dynamic changes of
lipids in cells, tissues or body fluids are investigated. Due to the vital roles of lipids in human …

Applications of mass spectrometry for cellular lipid analysis

C Wang, M Wang, X Han - Molecular BioSystems, 2015 - pubs.rsc.org
Mass spectrometric analysis of cellular lipids is an enabling technology for lipidomics, which
is a rapidly-developing research field. In this review, we briefly discuss the principles …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

DJ Harvey - Mass Spectrometry Reviews, 2018 - Wiley Online Library
This review is the eighth update of the original article published in 1999 on the application of
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry …

[HTML][HTML] Application of a novel chemical assay for the quantification of endotoxins in bacterial bioreactor samples

A Hoffmann, K Pacios, R Mühlemann… - … of Chromatography B, 2023 - Elsevier
A novel chemical assay, the so-called Kdo-DMB-liquid chromatography (LC) assay, was
used for the accurate and cost-effective determination of the endotoxin content in …

Glycan and Protein Analysis of Glycoengineered Bacterial E. coli Vaccines by MALDI-in-Source Decay FT-ICR Mass Spectrometry

S Nicolardi, R Danuser, V Dotz… - Analytical …, 2022 - ACS Publications
Bacterial glycoconjugate vaccines have a major role in preventing microbial infections.
Immunogenic bacterial glycans, such as O-antigen polysaccharides, can be recombinantly …

193 nm ultraviolet photodissociation mass spectrometry for the structural elucidation of lipid A compounds in complex mixtures

JP O'Brien, BD Needham, JC Henderson… - Analytical …, 2014 - ACS Publications
Here we implement ultraviolet photodissociation (UVPD) in an online liquid
chromatographic tandem mass spectrometry (MS/MS) strategy to support analysis of …