The annotation of small molecules remains a major challenge in untargeted mass
spectrometry-based metabolomics. We here critically discuss structured elucidation …

Peptides are biopolymers, typically consisting of 2–50 amino acids. They are biologically
produced by the cellular ribosomal machinery or by non-ribosomal enzymes and …

A growing appreciation of the importance of cellular metabolism and revelations concerning
the extent of cell–cell heterogeneity demand metabolic characterization of individual cells …

Glioblastomas are malignant tumors of the central nervous system hallmarked by subclonal
diversity and dynamic adaptation amid developmental hierarchies. The source of dynamic …

Untargeted metabolomics experiments rely on spectral libraries for structure annotation, but,
typically, only a small fraction of spectra can be matched. Previous in silico methods search …

Spatial omics technologies generate wealthy but highly complex datasets. Here we present
Spatial Omics DataBase (SODB), a web-based platform providing both rich data resources …

Matrix-assisted laser desorption–ionization mass spectrometry imaging in transmission-
mode geometry (t-MALDI–MSI) can provide molecular information with a pixel size of 1 µm …

Energetic metabolism reprogramming is critical for cancer and immune responses. Current
methods to functionally profile the global metabolic capacities and dependencies of cells are …

Highlights•Ideal mass spectrometer for MALDI MSI would satisfy the '4S-criteria for
performance'(speed, specificity, spatial resolution and sensitivity).•Quantification MALDI MSI …

Characterization of tumor metabolism with spatial information contributes to our
understanding of complex cancer metabolic reprogramming, facilitating the discovery of …