Paramagnetic chemical probes for studying biological macromolecules

Q Miao, C Nitsche, H Orton, M Overhand… - Chemical …, 2022 - ACS Publications
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Synthesis of nucleobase-modified RNA oligonucleotides by post-synthetic approach

K Bartosik, K Debiec, A Czarnecka, E Sochacka… - Molecules, 2020 - mdpi.com
The chemical synthesis of modified oligoribonucleotides represents a powerful approach to
study the structure, stability, and biological activity of RNAs. Selected RNA modifications …

Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution

S Ghosh, MJ Lawless, HJ Brubaker… - Nucleic acids …, 2020 - academic.oup.com
Electron paramagnetic resonance (EPR) has become an important tool to probe
conformational changes in nucleic acids. An array of EPR labels for nucleic acids are …

EPR Distance Measurements on Long Non‐coding RNAs Empowered by Genetic Alphabet Expansion Transcription

C Domnick, F Eggert, C Wuebben… - Angewandte …, 2020 - Wiley Online Library
We present herein a novel nitroxide spin label‐containing RNA triphosphate TPT3NO and
its application for site‐specific spin‐labeling of RNA through in vitro transcription using an …

2′-Alkynylnucleotides: a sequence-and spin label-flexible strategy for EPR spectroscopy in DNA

MM Haugland, AH El-Sagheer, RJ Porter… - Journal of the …, 2016 - ACS Publications
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate
molecular structure through the measurement of distances between conformationally well …

Development and application of spin traps, spin probes, and spin labels

EG Bagryanskaya, OA Krumkacheva, MV Fedin… - Methods in …, 2015 - Elsevier
This chapter focuses on major achievements of the last decade in the synthesis and
applications of spin traps, spin probes, and spin labels. Our discussion on spin trapping is …

Complementary-addressed site-directed spin labeling of long natural RNAs

ES Babaylova, AA Malygin, AA Lomzov… - Nucleic acids …, 2016 - academic.oup.com
Nanoscale distance measurements by pulse dipolar Electron paramagnetic resonance
(EPR) spectroscopy allow new insights into the structure and dynamics of complex …

Post‐synthetic Spin‐Labeling of RNA through Click Chemistry for PELDOR Measurements

M Kerzhner, D Abdullin, J Więcek… - … A European Journal, 2016 - Wiley Online Library
Site‐directed spin labeling of RNA based on click chemistry is used in combination with
pulsed electron‐electron double resonance (PELDOR) to benchmark a nitroxide spin label …

A versatile approach to attachment of triarylmethyl labels to DNA for nanoscale structural EPR studies at physiological temperatures

GY Shevelev, EL Gulyak, AA Lomzov… - The Journal of …, 2018 - ACS Publications
Triarylmethyl (trityl, TAM) radicals are a promising class of spin labels for nanometer-scale
distance measurements in biomolecules at physiological temperatures. However, to date …

Tuning the properties of nitroxide spin labels for use in electron paramagnetic resonance spectroscopy through chemical modification of the nitroxide framework

MM Haugland, EA Anderson, JE Lovett - 2016 - books.rsc.org
Spin labels containing nitroxyl radicals possess many properties that render them useful for
electron paramagnetic resonance (EPR) spectroscopy. This review describes the …