Paramagnetic chemical probes for studying biological macromolecules
Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …
Synthesis of nucleobase-modified RNA oligonucleotides by post-synthetic approach
K Bartosik, K Debiec, A Czarnecka, E Sochacka… - Molecules, 2020 - mdpi.com
The chemical synthesis of modified oligoribonucleotides represents a powerful approach to
study the structure, stability, and biological activity of RNAs. Selected RNA modifications …
study the structure, stability, and biological activity of RNAs. Selected RNA modifications …
Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution
S Ghosh, MJ Lawless, HJ Brubaker… - Nucleic acids …, 2020 - academic.oup.com
Electron paramagnetic resonance (EPR) has become an important tool to probe
conformational changes in nucleic acids. An array of EPR labels for nucleic acids are …
conformational changes in nucleic acids. An array of EPR labels for nucleic acids are …
EPR Distance Measurements on Long Non‐coding RNAs Empowered by Genetic Alphabet Expansion Transcription
C Domnick, F Eggert, C Wuebben… - Angewandte …, 2020 - Wiley Online Library
We present herein a novel nitroxide spin label‐containing RNA triphosphate TPT3NO and
its application for site‐specific spin‐labeling of RNA through in vitro transcription using an …
its application for site‐specific spin‐labeling of RNA through in vitro transcription using an …
2′-Alkynylnucleotides: a sequence-and spin label-flexible strategy for EPR spectroscopy in DNA
MM Haugland, AH El-Sagheer, RJ Porter… - Journal of the …, 2016 - ACS Publications
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate
molecular structure through the measurement of distances between conformationally well …
molecular structure through the measurement of distances between conformationally well …
Development and application of spin traps, spin probes, and spin labels
This chapter focuses on major achievements of the last decade in the synthesis and
applications of spin traps, spin probes, and spin labels. Our discussion on spin trapping is …
applications of spin traps, spin probes, and spin labels. Our discussion on spin trapping is …
Complementary-addressed site-directed spin labeling of long natural RNAs
ES Babaylova, AA Malygin, AA Lomzov… - Nucleic acids …, 2016 - academic.oup.com
Nanoscale distance measurements by pulse dipolar Electron paramagnetic resonance
(EPR) spectroscopy allow new insights into the structure and dynamics of complex …
(EPR) spectroscopy allow new insights into the structure and dynamics of complex …
Post‐synthetic Spin‐Labeling of RNA through Click Chemistry for PELDOR Measurements
M Kerzhner, D Abdullin, J Więcek… - … A European Journal, 2016 - Wiley Online Library
Site‐directed spin labeling of RNA based on click chemistry is used in combination with
pulsed electron‐electron double resonance (PELDOR) to benchmark a nitroxide spin label …
pulsed electron‐electron double resonance (PELDOR) to benchmark a nitroxide spin label …
A versatile approach to attachment of triarylmethyl labels to DNA for nanoscale structural EPR studies at physiological temperatures
GY Shevelev, EL Gulyak, AA Lomzov… - The Journal of …, 2018 - ACS Publications
Triarylmethyl (trityl, TAM) radicals are a promising class of spin labels for nanometer-scale
distance measurements in biomolecules at physiological temperatures. However, to date …
distance measurements in biomolecules at physiological temperatures. However, to date …
Tuning the properties of nitroxide spin labels for use in electron paramagnetic resonance spectroscopy through chemical modification of the nitroxide framework
MM Haugland, EA Anderson, JE Lovett - 2016 - books.rsc.org
Spin labels containing nitroxyl radicals possess many properties that render them useful for
electron paramagnetic resonance (EPR) spectroscopy. This review describes the …
electron paramagnetic resonance (EPR) spectroscopy. This review describes the …