Cancer metastasis is no longer viewed as a linear cascade of events but rather as a series
of concurrent, partially overlapping processes, as successfully metastasizing cells assume …

As a review for the 20th anniversary of publishing the antigen retrieval (AR) technique in this
journal, the authors intend briefly to summarize developments in AR-immunohistochemistry …

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major
bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling …

Abstract Analysis of the sequence and nature of protein N termini has many applications.
Defining the termini of proteins for proteome annotation in the Human Proteome Project is of …

Prostate cancer cells with stem cell characteristics were identified in human prostate cancer
cell lines by their ability to form from single cells self-renewing prostaspheres in non …

A novel method for highthroughput proteomic analysis of formalinfixed paraffinembedded
(FFPE) tissue microarrays (TMA) is described using ontissue tryptic digestion followed by …

Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable
resource for retrospective biomarker discovery studies, and recent work indicates the …

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the most effective tumor
immunotherapy available. Although allo-HSCT provides beneficial graft-versus-tumor …

Biobanks contain biological samples and associated information that are essential raw
materials for advancement of biotechnology, human health, and research and development …

Plasma membrane proteins that are exposed on the cell surface have important biological
functions, such as signaling into and out of the cells, ion transport, and cell-cell and cell …