Software-based image analysis is a crucial step in the biological interpretation of two-
dimensional gel electrophoresis experiments. Recent significant advances in image …

DIGE is a protein labelling and separation technique allowing quantitative proteomics of two
or more samples by optical fluorescence detection of differentially labelled proteins that are …

We assess the reliability of isobaric-tags for relative and absolute quantitation (iTRAQ),
based on different types of replicate analyses taking into account technical, experimental …

In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting
new technology has been developed and enthusiastically applied to an enormous variety of …

This review recollects literature data on sensitivity and dynamic range for the most
commonly used colorimetric and fluorescent dyes for general protein staining, and …

Red and green are two of the most‐preferred colors from the entire chromatic spectrum, and
red and green dyes are widely used in biochemistry, immunohistochemistry, immune …

Given the rapid developments in mass spectrometry (MS) in terms of sensitivity, mass
accuracy, and throughput, some have suggested that two-dimensional gel electrophoresis …

This review provides an introduction for the nonspecialist to proteomics and in particular the
major approaches available for global protein identification and quantification. Proteomics …

Two dimensional gel electrophoresis has been one of the techniques most used for protein
separation in proteomics experiments and still continues to be so for some species such as …

Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D
electrophoresis (2DE) that allows one to compare two or three protein samples …