Fluorescence-based protein stability monitoring—a review

N Gooran, K Kopra - International Journal of Molecular Sciences, 2024 - mdpi.com
Proteins are large biomolecules with a specific structure that is composed of one or more
long amino acid chains. Correct protein structures are directly linked to their correct function …

Some critical aspects of the enzymatic hydrolysis at high dry‐matter content: a review

F Battista, D Bolzonella - Biofuels, Bioproducts and Biorefining, 2018 - Wiley Online Library
Enzymatic hydrolysis represents the crucial phase in bioethanol production. Its aim is to
convert pretreated lignocellulosic polymers in hexose sugars for fermentation into …

Unveiling the potential of deep eutectic solvents to improve the conformational and colloidal stability of immunoglobulin G antibodies

D Dhiman, ASC Marques, M Bisht, APM Tavares… - Green …, 2023 - pubs.rsc.org
Antibodies, such as immunoglobulin G (IgG), have a pivotal role as biopharmaceuticals
capable of treating a wide variety of diseases. However, IgG is a protein, hence, it can easily …

Temperature responses of the Rubisco maximum carboxylase activity across domains of life: phylogenetic signals, trade-offs, and importance for carbon gain

J Galmes, MV Kapralov, LO Copolovici… - Photosynthesis …, 2015 - Springer
Abstract Temperature response of ribulose-1, 5-bisphosphate carboxylase/oxygenase
(Rubisco) catalytic properties directly determines the CO 2 assimilation capacity of …

Extraction of thermodynamic parameters of protein unfolding using parallelized differential scanning fluorimetry

TA Wright, JM Stewart, RC Page… - The journal of physical …, 2017 - ACS Publications
Thermodynamic properties of protein unfolding have been extensively studied; however, the
methods used have typically required significant preparation time and high protein …

Ionic liquid-based strategy for predicting protein aggregation propensity and thermodynamic stability

TA Shmool, LK Martin, RP Matthews, JP Hallett - Jacs Au, 2022 - ACS Publications
Novel drug candidates are continuously being developed to combat the most life-
threatening diseases; however, many promising protein therapeutics are dropped from the …

Biochemical and Thermodynamic Studies on a Novel Thermotolerant GH10 Xylanase from Bacillus safensis

PD Glekas, S Kalantzi, A Dalios, DG Hatzinikolaou… - Biomolecules, 2022 - mdpi.com
Xylanases have a broad range of applications in agro-industrial processes. In this study, we
report on the discovery and characterization of a new thermotolerant GH10 xylanase from …

Production of bio-hydrogen from dairy wastewater using pretreated landfill leachate sludge as an inoculum

YM Wong, PL Show, TY Wu, HY Leong… - Journal of bioscience …, 2019 - Elsevier
Highlights•Utilized landfill leachate sludge inoculum for H 2 production from dairy
wastewater.•The maximum H 2 yield was 113.2±2.9 mmol H 2/g COD.•Utilized kinetic …

Identification and computational analysis of mutations in SARS-CoV-2

T Dey, S Chatterjee, S Manna, A Nandy… - Computers in biology and …, 2021 - Elsevier
SARS-CoV-2 infection has become a worldwide pandemic and is spreading rapidly to
people across the globe. To combat the situation, vaccine design is the essential solution …

pH-Dependent aggregation and disaggregation of native β-lactoglobulin in low salt

Y Yan, D Seeman, B Zheng, E Kizilay, Y Xu, PL Dubin - Langmuir, 2013 - ACS Publications
The aggregation of β-lactoglobulin (BLG) near its isoelectric point was studied as a function
of ionic strength and pH. We compared the behavior of native BLG with those of its two …