A beginner's guide to low‐coverage whole genome sequencing for population genomics
Low‐coverage whole genome sequencing (lcWGS) has emerged as a powerful and cost‐
effective approach for population genomic studies in both model and nonmodel species …
effective approach for population genomic studies in both model and nonmodel species …
DuBA. flow─ A Low-Cost, Long-Read Amplicon Sequencing Workflow for the Validation of Synthetic DNA Constructs
AA Ramírez Rojas, CK Brinkmann… - ACS Synthetic …, 2024 - ACS Publications
Modern biological science, especially synthetic biology, relies heavily on the construction of
DNA elements, often in the form of plasmids. Plasmids are used for a variety of applications …
DNA elements, often in the form of plasmids. Plasmids are used for a variety of applications …
[HTML][HTML] The cytidine deaminase APOBEC3C has unique sequence and genome feature preferences
GW Brown - Genetics, 2024 - academic.oup.com
APOBEC proteins are cytidine deaminases that restrict the replication of viruses and
transposable elements. Several members of the APOBEC3 family, APOBEC3A, APOBEC3B …
transposable elements. Several members of the APOBEC3 family, APOBEC3A, APOBEC3B …
[HTML][HTML] High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase …
L Cobb, E de Muinck, S Kollias, M Skage, GD Gilfillan… - Plos one, 2024 - journals.plos.org
Entomological sampling and storage conditions often prioritise efficiency, practicality and
conservation of morphological characteristics, and may therefore be suboptimal for DNA …
conservation of morphological characteristics, and may therefore be suboptimal for DNA …
[HTML][HTML] Evaluating coverage bias in next-generation sequencing of Escherichia coli
Whole-genome sequencing is essential to many facets of infectious disease research.
However, technical limitations such as bias in coverage and tagmentation, and difficulties …
However, technical limitations such as bias in coverage and tagmentation, and difficulties …
Validation of Golden Gate assemblies using highly multiplexed Nanopore amplicon sequencing
AAR Rojas, CK Brinkmann, D Schindler - arXiv preprint arXiv:2401.14191, 2024 - arxiv.org
Golden Gate cloning has revolutionized synthetic biology. Its concept of modular, highly
characterized libraries of parts that can be combined into higher order assemblies allows …
characterized libraries of parts that can be combined into higher order assemblies allows …
[PDF][PDF] Ανάπτυξη ενός πιο οικονομικού και αποδοτικού πρωτοκόλλου για την αλληλούχηση μοναδιαίων βακτηριακών κυττάρων
ΒΑ Μιχελιουδάκης - 2022 - ir.lib.uth.gr
Αναλύσεις μη καλλιεργήσιμων βακτηριακών κοινοτήτων με τεχνολογίες μαζικής
αλληλούχησης, όπως αλληλούχηση τυχαίας προσπέλασης, χρησιμοποιούνται ευρέως για τον …
αλληλούχησης, όπως αλληλούχηση τυχαίας προσπέλασης, χρησιμοποιούνται ευρέως για τον …
Comparison of Nextera XT and Collibri ES library preparation kits: from wet lab to bioinformatics analysis
L Nassar-Míguez, A Flores-Cruz… - 2022 IEEE 4th …, 2022 - ieeexplore.ieee.org
Whole genome sequencing (WGS) analyses are key for a great number of studies
nowadays. In order to carry out WGS, DNA is fragmented and adapter sequences are added …
nowadays. In order to carry out WGS, DNA is fragmented and adapter sequences are added …
Scalable approaches for gene tagging and genome walking sequencing
K Herbst - 2021 - archiv.ub.uni-heidelberg.de
A central question in the life sciences is how an observed phenotype is realized by a given
biological system. One explanatory factor is certainly the genotype of such a system which …
biological system. One explanatory factor is certainly the genotype of such a system which …