Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Industrial biotechnology and microbial metabolic engineering are poised to help meet the
growing demand for sustainable, low‐cost commodity chemicals and natural products, yet …

The ability to build synthetic cellular populations from the bottom-up provides the
groundwork to realize minimal living tissues comprising single cells which can communicate …

Cell-free synthetic biology is emerging as a powerful approach aimed to understand,
harness, and expand the capabilities of natural biological systems without using intact cells …

Speeding up design-build-test (DBT) cycles is a fundamental challenge facing biochemical
engineering. To address this challenge, we report a new cell-free protein synthesis driven …

In vitro enzyme cascades possess great benefits, such as their synthetic capabilities for
complex molecules, no need for intermediate isolation, and the shift of unfavorable equilibria …

Metabolic engineering efforts that harness living organisms to produce natural products and
other useful chemicals face inherent difficulties because the maintenance of life processes …

Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …

Synthetic biology concerns the engineering of man‐made living biomachines from
standardized components that can perform predefined functions in a (self‐) controlled …

Abstract High-energy-density, green, safe batteries are highly desirable for meeting the
rapidly growing needs of portable electronics. The incomplete oxidation of sugars mediated …