Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
A journey with psychedelic mushrooms: From historical relevance to biology, cultivation, medicinal uses, biotechnology, and beyond
Psychedelic mushrooms containing psilocybin and related tryptamines have long been used
for ethnomycological purposes, but emerging evidence points to the potential therapeutic …
for ethnomycological purposes, but emerging evidence points to the potential therapeutic …
Advantages of supports activated with divinyl sulfone in enzyme coimmobilization: Possibility of multipoint covalent immobilization of the most stable enzyme and …
R Morellon-Sterling, D Carballares… - ACS Sustainable …, 2021 - ACS Publications
Vinyl sulfone (VS)–agarose beads were used to develop a new strategy to coimmobilize
enzymes with very different stabilities, enabling the reuse of the most stable immobilized …
enzymes with very different stabilities, enabling the reuse of the most stable immobilized …
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances
X Wei, Z Chen, A Liu, L Yang, Y Xu, M Cao… - Biotechnology Advances, 2023 - Elsevier
Extracellular polymeric substances are mainly synthesized via a variety of biosynthetic
pathways in bacteria. Bacilli-sourced extracellular polymeric substances, such as …
pathways in bacteria. Bacilli-sourced extracellular polymeric substances, such as …
Prolonging genetic circuit stability through adaptive evolution of overlapping genes
The development of synthetic biological circuits that maintain functionality over application-
relevant time scales remains a significant challenge. Here, we employed synthetic …
relevant time scales remains a significant challenge. Here, we employed synthetic …
Directed genome evolution driven by structural rearrangement techniques
S Zhou, Y Wu, ZX Xie, B Jia, YJ Yuan - Chemical Society Reviews, 2021 - pubs.rsc.org
Directed genome evolution simulates the process of natural evolution at the genomic level in
the laboratory to generate desired phenotypes. Here we review the applications of recent …
the laboratory to generate desired phenotypes. Here we review the applications of recent …
Advances in Metabolic Engineering of Saccharomyces cerevisiae for Cocoa Butter Equivalent Production
Cocoa butter is extracted from cocoa beans, and it is mainly used as the raw material for the
production of chocolate and cosmetics. Increased demands and insufficient cocoa plants led …
production of chocolate and cosmetics. Increased demands and insufficient cocoa plants led …
[HTML][HTML] Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads
Trypsin, chymotrypsin, penicillin G acylase and ficin extract have been stabilized by
immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary …
immobilization on glyoxyl agarose, adding different aliphatic compounds bearing a primary …
Engineering robust microorganisms for organic acid production
Organic acids are an important class of compounds that can be produced by microbial
conversion of renewable feedstocks and have huge demands and broad applications in …
conversion of renewable feedstocks and have huge demands and broad applications in …
Identification of novel metabolic engineering targets for S-adenosyl-L-methionine production in Saccharomyces cerevisiae via genome-scale engineering
C Dong, JC Schultz, W Liu, J Lian, L Huang, Z Xu… - Metabolic …, 2021 - Elsevier
Abstract S-Adenosyl-L-methionine (SAM) is an important intracellular metabolite and widely
used for treatment of various diseases. Although high level production of SAM had been …
used for treatment of various diseases. Although high level production of SAM had been …