Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry

JD Dunn, GE Reid, ML Bruening - Mass spectrometry reviews, 2010 - Wiley Online Library
Mass spectrometry is the tool of choice to investigate protein phosphorylation, which plays a
vital role in cell regulation and diseases such as cancer. However, low abundances of …

Phosphopeptide enrichment using metal oxide affinity chromatography

A Leitner - TrAC Trends in Analytical Chemistry, 2010 - Elsevier
The demand for effective sample preparation strategies that enable the in-depth study of
protein phosphorylation has spawned a number of selective affinity techniques that allow …

Recent advances in phosphopeptide enrichment: strategies and techniques

XS Li, BF Yuan, YQ Feng - TrAC Trends in Analytical Chemistry, 2016 - Elsevier
Phosphoproteomics has become one of the most active research areas in proteomics
studies. Phosphopeptide enrichment is a critical and indispensable step in …

Ordered mesoporous alumina with ultra-large pores as an efficient absorbent for selective bioenrichment

J Wei, Y Ren, W Luo, Z Sun, X Cheng, Y Li… - Chemistry of …, 2017 - ACS Publications
Alumina has recently turned out to be effective in enrichment of biomacrolecules like
phosphopeptides due to its good affinity to phosphor groups. Ordered mesoporous alumina …

Metal oxides in sample pretreatment

J Xu, P Wu, EC Ye, BF Yuan, YQ Feng - TrAC Trends in Analytical …, 2016 - Elsevier
Sample pretreatment is a fundamental and essential step in almost all analytical procedures,
especially for the analysis of biological and environmental samples with complex matrix. In …

Enrichment of phosphorylated peptides with metal–organic framework nanosheets for serum profiling of diabetes and phosphoproteomics analysis

SS Yang, YJ Chang, H Zhang, X Yu, W Shang… - Analytical …, 2018 - ACS Publications
Capturing phosphopeptides from complicated biological samples is essential for the
discovery of new post-translational modification sites and disease diagnostics. Although …

Development of phosphopeptide enrichment techniques for phosphoproteome analysis

G Han, M Ye, H Zou - Analyst, 2008 - pubs.rsc.org
Protein phosphorylation is one of the most biologically relevant and ubiquitous post-
translational modifications. The analysis of protein phosphorylation is very challenging due …

A poly (ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides

L Zhao, H Qin, Z Hu, Y Zhang, H Zou - Chemical Science, 2012 - pubs.rsc.org
Immobilized metal affinity chromatography (IMAC) is a powerful method in phosphopeptide
enrichment. However, the achievement of highly specific enrichment and sensitive detection …

On‐plate‐selective enrichment of glycopeptides using boronic acid‐modified gold nanoparticles for direct MALDI‐QIT‐TOF MS analysis

J Tang, Y Liu, D Qi, G Yao, C Deng, X Zhang - Proteomics, 2009 - Wiley Online Library
In this study, an on‐plate‐selective enrichment method is developed for fast and efficient
glycopeptide investigation. Gold nanoparticles were first spotted and sintered on a stainless …

Coupling strong anion-exchange monolithic capillary with MALDI-TOF MS for sensitive detection of phosphopeptides in protein digest

M Dong, M Wu, F Wang, H Qin, G Han, J Dong… - Analytical …, 2010 - ACS Publications
Protein phosphorylation is one of the most biologically relevant and ubiquitous post-
translational modifications. Matrix-assisted laser desorption ionization time-of-flight mass …