The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

Molecular inputs to chromatin via cellular metabolism are modifiers of the epigenome. These
inputs—which include both nutrient availability as a result of diet and growth factor signalling …

A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

It remains unclear why acute depletion of CTCF (CCCTC-binding factor) and cohesin only
marginally affects expression of most genes despite substantially perturbing three …

Single-cell RNA sequencing has revealed extensive transcriptional cell state diversity in
cancer, often observed independently of genetic heterogeneity, raising the central question …

Human gene expression is regulated by more than 2,000 transcription factors and chromatin
regulators,. Effector domains within these proteins can activate or repress transcription …

Heterochromatin is a key architectural feature of eukaryotic chromosomes, which endows
particular genomic domains with specific functional properties. The capacity of …

To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

Somatic mutations in cancer genes have been detected in clonal expansions across healthy
human tissue, including in clonal hematopoiesis. However, because mutated and wild-type …