[HTML][HTML] Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been developing since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodríguez, R Martínez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Importance of the support properties for immobilization or purification of enzymes

JCS Santos, O Barbosa, C Ortiz… - …, 2015 - Wiley Online Library
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

Recent advances in electrochemical glucose biosensors: a review

C Chen, Q Xie, D Yang, H Xiao, Y Fu, Y Tan, S Yao - Rsc Advances, 2013 - pubs.rsc.org
Glucose detection is of great significance in biomedical applications. Principles, methods
and recent developments in electrochemical glucose sensors are reviewed here. Special …

[HTML][HTML] Agarose and its derivatives as supports for enzyme immobilization

P Zucca, R Fernandez-Lafuente, E Sanjust - Molecules, 2016 - mdpi.com
Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …

Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties

O Barbosa, R Torres, C Ortiz… - …, 2013 - ACS Publications
A heterofunctional support for enzyme immobilization may be defined as that which
possesses several distinct functionalities on its surface able to interact with a protein. We will …

[HTML][HTML] Immobilization of papain: A review

VG Tacias-Pascacio, R Morellon-Sterling… - International Journal of …, 2021 - Elsevier
Papain is a cysteine protease from papaya, with many applications due to its broad
specificity. This paper reviews for first time the immobilization of papain on different supports …

Chemical modification in the design of immobilized enzyme biocatalysts: drawbacks and opportunities

N Rueda, JCS Dos Santos, C Ortiz… - The Chemical …, 2016 - Wiley Online Library
Chemical modification of enzymes and immobilization used to be considered as separate
ways to improve enzyme properties. This review shows how the coupled use of both tools …

[HTML][HTML] A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

R Morellon-Sterling, O Tavano, JM Bolivar… - International journal of …, 2022 - Elsevier
Pepsin is a protease used in many different applications, and in many instances, it is utilized
in an immobilized form to prevent contamination of the reaction product. This enzyme has …