Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …

Natural products, a chemically and structurally diverse class of molecules, possess a wide
spectrum of biological activities, have been used therapeutically for millennia, and have …

By exploiting versatile P450 enzymes, whole‐cell biocatalysis can be performed to
synthesize valuable compounds in Escherichia coli. However, the insufficient supply of …

The production of membrane proteins of high purity and in satisfactory yields is crucial for
biomedical research. Due to their involvement in various cellular processes, membrane …

Controlled gene expression is fundamental for the study of gene function and our ability to
engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables …

Gene expression toxicity is an important biological phenomenon and a major bottleneck in
biotechnology. Escherichia coli BL21 (DE3) is the most popular choice for recombinant …

The uptake of fluorescently labeled ultrasmall gold nanoparticles (2 nm) by Gram‐negative
Escherichia coli bacteria occurs within 1–3 hours. This was demonstrated by confocal laser …

Overexpression of correctly folded membrane proteins is a fundamental prerequisite for
functional and structural studies. One of the most commonly used expression systems for the …

Simple Summary Membrane proteins are essential to all forms of life. Millions of membrane
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …

Controlled gene expression is crucial for engineering bacteria for basic and applied
research. Inducible systems enable tight regulation of expression, wherein a small-molecule …