Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

[HTML][HTML] Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D

J Park, L Gao - Current Opinion in Solid State and Materials Science, 2024 - Elsevier
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering
molecular specific insights into samples through the measurement of fluorescence decay …

Single-sample image-fusion upsampling of fluorescence lifetime images

V Kapitany, A Fatima, V Zickus, J Whitelaw… - Science …, 2024 - science.org
Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about
molecular interactions and biological processes. A major bottleneck for FLIM is image …

Learned Compressive Representations for Single-Photon 3D Imaging

F Gutierrez-Barragan, F Mu… - Proceedings of the …, 2023 - openaccess.thecvf.com
Single-photon 3D cameras can record the time-of-arrival of billions of photons per second
with picosecond accuracy. One common approach to summarize the photon data stream is …

A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM …

J Nedbal, F Mattioli Della Rocca, IT Ivanova, A Allan… - Scientific Reports, 2024 - nature.com
A wide-field microscope with epi-fluorescence and selective plane illumination was
combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell …

Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM

B Shen, Y Lu, F Guo, F Lin, R Hu, F Rao, J Qu… - Communications …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) provides quantitative readouts of
biochemical microenvironments, holding great promise for biomedical imaging. However …

Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy

DL Pham, D Cappabianca, M Forsberg, C Weaver… - bioRxiv, 2024 - biorxiv.org
Chimeric antigen receptor (CAR) T cell therapy for solid tumors remains challenging due to
the complex manufacturing process and the immunosuppressive tumor microenvironment …

A Review of Image Sensors Used in Near-Infrared and Shortwave Infrared Fluorescence Imaging

B Zhu, H Jonathan - Sensors, 2024 - mdpi.com
To translate near-infrared (NIR) and shortwave infrared (SWIR) fluorescence imaging into
the clinic, the paired imaging device needs to detect trace doses of fluorescent imaging …

Perspectives on label-free microscopy of heterogeneous and dynamic biological systems

DL Pham, AA Gillette, J Riendeau… - Journal of …, 2025 - spiedigitallibrary.org
Significance Advancements in label-free microscopy could provide real-time, non-invasive
imaging with unique sources of contrast and automated standardized analysis to …

High-efficiency digitally scanned light-sheet fluorescence lifetime microscopy (DSLM-FLIM)

KJ Nutt, D Olesker, E McGhee, G Hungerford… - bioRxiv, 2023 - biorxiv.org
Recently-developed kilopixel single-photon avalanche diode (SPAD) arrays with in-pixel
timing hold great promise for fluorescence lifetime imaging microscopy of dynamic samples …