Beyond the genetic code, the human proteome is greatly diversified by genetic variations,
alternative splicing of RNA transcripts, and post-translational modifications (PTMs). 6, 7 In …

The analysis of peptides and proteins as well as the grander scope of proteomics (large
scale study of proteins) has been advanced by the development of a versatile array of ion …

Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …

Capillary electrophoresis (CE) is a field that continues to grow. All areas of CE including
theory, separation modes, instrumentation, and applications remain active areas of …

Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been
recognized as a useful tool for top-down proteomics. However, its performance for deep top …

Mass spectrometry (MS)-based top-down proteomics characterizes complex proteomes at
the intact proteoform level and provides an accurate picture of protein isoforms and protein …

Here, we demonstrate an integrated microfluidic capillary electrophoresis-electrospray
ionization (CE-ESI) device for the separation of intact monoclonal antibody charge variants …

Top-down ultraviolet photodissociation (UVPD) allows greater sequence coverage than any
other currently available method, often fracturing the vast majority of peptide bonds in whole …

Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-
MS/MS) has been recognized as an invaluable platform for top-down proteomics. However …

Top‐down proteomics is emerging as a preferred approach to investigate biological
systems, with objectives ranging from the detailed assessment of a single protein …