CRISPR screens in plants: approaches, guidelines, and future prospects
C Gaillochet, W Develtere, TB Jacobs - The Plant Cell, 2021 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems
have revolutionized genome engineering by facilitating a wide range of targeted DNA …
have revolutionized genome engineering by facilitating a wide range of targeted DNA …
Large-scale genome editing in plants: approaches, applications, and future perspectives
T Liu, X Zhang, K Li, Q Yao, D Zhong, Q Deng… - Current Opinion in …, 2023 - Elsevier
Highlights•Presented the typical workflow of a CRISPR screen in plants.•Collected and
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Hundreds of human genes are associated with neurological diseases, but translation into
tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to …
tractable biological mechanisms is lagging. Larval zebrafish are an attractive model to …
The APOE-R136S mutation protects against APOE4-driven Tau pathology, neurodegeneration and neuroinflammation
Abstract Apolipoprotein E4 (APOE4) is the strongest genetic risk factor for late-onset
Alzheimer's disease (LOAD), leading to earlier age of clinical onset and exacerbating …
Alzheimer's disease (LOAD), leading to earlier age of clinical onset and exacerbating …
[HTML][HTML] Embryo-scale reverse genetics at single-cell resolution
The maturation of single-cell transcriptomic technologies has facilitated the generation of
comprehensive cellular atlases from whole embryos,,–. A majority of these data, however …
comprehensive cellular atlases from whole embryos,,–. A majority of these data, however …
Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing
S Wimberger, N Akrap, M Firth, J Brengdahl… - Nature …, 2023 - nature.com
Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical
research and offers potential cures for genetic diseases. Despite rapid progress, low …
research and offers potential cures for genetic diseases. Despite rapid progress, low …
Global detection of DNA repair outcomes induced by CRISPR–Cas9
CRISPR–Cas9 generates double-stranded DNA breaks (DSBs) to activate cellular DNA
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
repair pathways for genome editing. The repair of DSBs leads to small insertions or …
A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies
L Hoberecht, P Perampalam, A Lun… - Nature Communications, 2022 - nature.com
The success of CRISPR-mediated gene perturbation studies is highly dependent on the
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
Efficient and safe therapeutic use of paired Cas9-nickases for primary hyperoxaluria type 1
L Torella, J Klermund, M Bilbao-Arribas… - EMBO Molecular …, 2024 - embopress.org
The therapeutic use of adeno-associated viral vector (AAV)-mediated gene disruption using
CRISPR-Cas9 is limited by potential off-target modifications and the risk of uncontrolled …
CRISPR-Cas9 is limited by potential off-target modifications and the risk of uncontrolled …