Differential Effects of Sequence-Local versus Nonlocal Charge Patterns on Phase Separation and Conformational Dimensions of Polyampholytes as Model …

T Pal, J Wessén, S Das, HS Chan - The Journal of Physical …, 2024 - ACS Publications
Conformational properties of intrinsically disordered proteins (IDPs) are governed by a
sequence-ensemble relationship. To differentiate the impact of sequence-local versus …

Nanocarriers for intracellular delivery of proteins in biomedical applications: strategies and recent advances

C Zhu, J Mu, L Liang - Journal of Nanobiotechnology, 2024 - Springer
Protein drugs are of great importance in maintaining the normal functioning of living
organisms. Indeed, they have been instrumental in combating tumors and genetic diseases …

[HTML][HTML] Amino acid-dependent phase equilibrium and material properties of tetrapeptide condensates

Y Zhang, R Prasad, S Su, D Lee, HX Zhou - Cell Reports Physical Science, 2024 - cell.com
The rules of how amino acids dictate the physical properties of biomolecular condensates
are still incomplete. Here, we study condensates formed by tetrapeptides of the form …

Direct and indirect salt effects on homotypic phase separation

M MacAinsh, S Dey, HX Zhou - eLife, 2024 - elifesciences.org
The low-complexity domain of hnRNPA1 (A1-LCD) phase separates in a salt-dependent
manner. Unlike many intrinsically disordered proteins (IDPs) whose phase separation is …

[HTML][HTML] In the Beginning: Let Hydration Be Coded in Proteins for Manifestation and Modulation by Salts and Adenosine Triphosphate

J Song - International Journal of Molecular Sciences, 2024 - pmc.ncbi.nlm.nih.gov
Water exists in the beginning and hydrates all matter. Life emerged in water, requiring three
essential components in compartmentalized spaces:(1) universal energy sources driving …

Mesoscale properties of biomolecular condensates emerging from protein chain dynamics

N Galvanetto, MT Ivanović, SA Del Grosso… - arXiv preprint arXiv …, 2024 - arxiv.org
Biomolecular condensates form by phase separation of biological polymers. The cellular
functions of the resulting membraneless organelles are closely linked to their physical …

[HTML][HTML] Amino Acid-Dependent Material Properties of Tetrapeptide Condensates

Y Zhang, R Prasad, S Su, D Lee, HX Zhou - bioRxiv, 2024 - ncbi.nlm.nih.gov
Condensates formed by intrinsically disordered proteins mediate a myriad of cellular
processes and are linked to pathological conditions including neurodegeneration. Rules of …

[HTML][HTML] Phase separation drives the folding of recombinant collagen

M Shen, D Astapov, D Fedorov, T Välisalmi… - International Journal of …, 2024 - Elsevier
Recombinantly produced collagens present a sustainable, ethical, and safe substitute for
collagens derived from natural sources. However, controlling the folding of the recombinant …

Direct and indirect salt effects on homotypic phase separation

MA Matt, D Souvik, Z Huan-Xiang - eLife, 2024 - search.proquest.com
The low-complexity domain of hnRNPA1 (A1-LCD) phase separates in a salt-dependent
manner. Unlike many intrinsically disordered proteins (IDPs) whose phase separation is …

Polyphosphate Discriminates Protein Conformational Ensembles More Efficiently than DNA Promoting Diverse Assembly and Maturation Behaviors

S Goyal, D Rajendran, AK Mani, AN Naganathan - bioRxiv, 2024 - biorxiv.org
Disordered proteins and domains often assemble into condensates with polyanionic nucleic
acids, primarily via charge complementarity, regulating numerous cellular functions …