Single‐strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are
popular electrophoretic methods for the identification of sequences. The principle reasons …

The highest sensitivity nucleic acid gel stains developed to date are optimally excited using
short-wavelength ultraviolet or visible light. This is a disadvantage for laboratories equipped …

Both benign and malignant tumors represent heterogenous tissue containing tumor cells
and non-neoplastic mesenchymal and inflammatory cells. To detect a minority of mutant …

Purpose: ras oncogenic activation has long been demonstrated in thyroid carcinomas of
follicular cell derivation, but no consistent relationship has been shown between mutations …

Background The development of targeted therapies has created a pressing clinical need for
the rapid and robust molecular characterisation of cancers. We describe here the application …

Several milestones in biology have been achieved since the first publication of the
Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true …

The identification of gene mutations is a critical goal for the assessment of diagnosis and
prognosis in cancer disease, particularly by direct sequencing. Pyrosequencing is a …

KRAS mutation testing before anti-epidermal growth factor receptor therapy of metastatic
colorectal cancer has become mandatory in Europe. However, considerable uncertainty …

BACKGROUND The identification of molecular alterations has an important therapeutic
implication in patients with lung adenocarcinomas. In the current study, the authors …

Activating mutation of KRAS plays a significant role in the pathogenesis of common human
malignancies and molecular testing of KRAS mutation has emerged as an essential …