The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay
detects DNA breakage by labeling the free 3ʹ-hydroxyl termini. Given that genomic DNA …

PARP7 is a monoPARP that catalyzes the transfer of single units of ADP-ribose onto
substrates to change their function. Here, we identify PARP7 as a negative regulator of …

DNA double-strand breaks (DSBs) are fundamental to cell biology, from evolution to the
latest gene-editing technologies. Yet, does an assay exist that truly quantitatively visualizes …

CRISPR Cas9-based screening is a powerful approach for identifying and characterizing
novel drug targets. Here, we elucidate the synthetic lethal mechanism of deubiquitinating …

Abstract sBLISS (in-suspension breaks labeling in situ and sequencing) is a versatile and
widely applicable method for identification of endogenous and induced DNA double-strand …

The untemplated activity of terminal deoxynucleotidyl transferase (TdT) represents its most
appealing feature. Its use is well established in applications aiming for extension of a DNA …

Accurately characterizing DNA double-stranded breaks (DSBs) and understanding the DNA
damage response (DDR) is crucial for assessing cellular genotoxicity, maintaining genomic …

In response to DNA double strand breaks (DSB), repair proteins accumulate at damaged
sites, forming membrane-less condensates or “foci”. The formation of these foci and their …

DNA damage is one of the foremost mechanisms of irradiation at the biological level. After
the first isolation of DNA by Friedrich Miescher in the 19th century, the structure of DNA was …

Age-related microangiopathy, also known as small vessel disease (SVD), causes damage to
the brain, retina, liver, and kidney. Based on the DNA damage theory of aging, we reasoned …