Quantum yield and brightness

KL Wong, JCG Bünzli, PA Tanner - Journal of Luminescence, 2020 - Elsevier
Brightness has different meaning in different contexts and some of these are reviewed. The
scientific definition of brightness does not represent our perception. The current definition is …

Biological phase separation and biomolecular condensates in plants

RJ Emenecker, AS Holehouse… - Annual review of plant …, 2021 - annualreviews.org
A surge in research focused on understanding the physical principles governing the
formation, properties, and function of membraneless compartments has occurred over the …

Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level

G Mekonnen, N Djaja, X Yuan, S Myong - Current Opinion in Chemical …, 2023 - Elsevier
Protein-protein and protein-RNA interactions are essential for cell function and survival.
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …

Determination of G-protein–coupled receptor oligomerization by molecular brightness analyses in single cells

A Işbilir, R Serfling, J Möller, R Thomas, C De Faveri… - Nature …, 2021 - nature.com
Oligomerization of membrane proteins has received intense research interest because of
their importance in cellular signaling and the large pharmacological and clinical potential …

The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor

A Litsios, P Goswami, HM Terpstra… - Molecular biology of …, 2022 - Am Soc Cell Biol
RESULTS AND DISCUSSION There is little or no change in Whi5 concentration during G1 It
was originally proposed by Schmoller et al.(2015) that Whi5 concentration decreases during …

Phase separation-mediated chromatin organization and dynamics: From imaging-based quantitative characterizations to functional implications

WS Ng, H Sielaff, ZW Zhao - International Journal of Molecular Sciences, 2022 - mdpi.com
As an effective and versatile strategy to compartmentalize cellular components without the
need for lipid membranes, phase separation has been found to underpin a wide range of …

Single-molecule detection-based super-resolution imaging in single-cell analysis: Inspiring progress and future prospects

Y Sun, Z Wu, Y Shang, SH Kang, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
Single-molecule detection-based super-resolution microscopy (SMDSRM) has enabled
researchers to analyze single-cells with resolution of colorful image in tens of nanometer …

Quantifying biomolecular organisation in membranes with brightness-transit statistics

F Schneider, PF Cespedes, N Karedla… - Nature …, 2024 - nature.com
Cells crucially rely on the interactions of biomolecules at their plasma membrane to maintain
homeostasis. Yet, a methodology to systematically quantify biomolecular organisation …

Absolute quantification of protein number and dynamics in single cells

CA Royer, M Tyers, S Tollis - Current Opinion in Structural Biology, 2023 - Elsevier
Quantitative characterization of protein abundance and interactions in live cells is necessary
to understand and predict cellular behavior. The accurate determination of copy number for …

CRDBP Protein Phase Separation and Its Recruitment to β-Catenin Protein in a Single Living Cell

J Liu, S Yu, W Yu, C Dong, X Huang… - The Journal of Physical …, 2023 - ACS Publications
The Coding Region Determinant-Binding Protein (CRDBP) is a carcinoembryonic protein,
and it is overexpressed in various cancer cells in the form of granules. We speculated the …