Quantum yield and brightness
Brightness has different meaning in different contexts and some of these are reviewed. The
scientific definition of brightness does not represent our perception. The current definition is …
scientific definition of brightness does not represent our perception. The current definition is …
Biological phase separation and biomolecular condensates in plants
RJ Emenecker, AS Holehouse… - Annual review of plant …, 2021 - annualreviews.org
A surge in research focused on understanding the physical principles governing the
formation, properties, and function of membraneless compartments has occurred over the …
formation, properties, and function of membraneless compartments has occurred over the …
Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level
Protein-protein and protein-RNA interactions are essential for cell function and survival.
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …
Determination of G-protein–coupled receptor oligomerization by molecular brightness analyses in single cells
A Işbilir, R Serfling, J Möller, R Thomas, C De Faveri… - Nature …, 2021 - nature.com
Oligomerization of membrane proteins has received intense research interest because of
their importance in cellular signaling and the large pharmacological and clinical potential …
their importance in cellular signaling and the large pharmacological and clinical potential …
The timing of Start is determined primarily by increased synthesis of the Cln3 activator rather than dilution of the Whi5 inhibitor
A Litsios, P Goswami, HM Terpstra… - Molecular biology of …, 2022 - Am Soc Cell Biol
RESULTS AND DISCUSSION There is little or no change in Whi5 concentration during G1 It
was originally proposed by Schmoller et al.(2015) that Whi5 concentration decreases during …
was originally proposed by Schmoller et al.(2015) that Whi5 concentration decreases during …
Phase separation-mediated chromatin organization and dynamics: From imaging-based quantitative characterizations to functional implications
As an effective and versatile strategy to compartmentalize cellular components without the
need for lipid membranes, phase separation has been found to underpin a wide range of …
need for lipid membranes, phase separation has been found to underpin a wide range of …
Single-molecule detection-based super-resolution imaging in single-cell analysis: Inspiring progress and future prospects
Y Sun, Z Wu, Y Shang, SH Kang, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
Single-molecule detection-based super-resolution microscopy (SMDSRM) has enabled
researchers to analyze single-cells with resolution of colorful image in tens of nanometer …
researchers to analyze single-cells with resolution of colorful image in tens of nanometer …
Quantifying biomolecular organisation in membranes with brightness-transit statistics
F Schneider, PF Cespedes, N Karedla… - Nature …, 2024 - nature.com
Cells crucially rely on the interactions of biomolecules at their plasma membrane to maintain
homeostasis. Yet, a methodology to systematically quantify biomolecular organisation …
homeostasis. Yet, a methodology to systematically quantify biomolecular organisation …
Absolute quantification of protein number and dynamics in single cells
Quantitative characterization of protein abundance and interactions in live cells is necessary
to understand and predict cellular behavior. The accurate determination of copy number for …
to understand and predict cellular behavior. The accurate determination of copy number for …
CRDBP Protein Phase Separation and Its Recruitment to β-Catenin Protein in a Single Living Cell
J Liu, S Yu, W Yu, C Dong, X Huang… - The Journal of Physical …, 2023 - ACS Publications
The Coding Region Determinant-Binding Protein (CRDBP) is a carcinoembryonic protein,
and it is overexpressed in various cancer cells in the form of granules. We speculated the …
and it is overexpressed in various cancer cells in the form of granules. We speculated the …