Murine single-cell RNA-seq reveals cell-identity-and tissue-specific trajectories of aging

JC Kimmel, L Penland, ND Rubinstein… - Genome …, 2019 - genome.cshlp.org
Aging is a pleiotropic process affecting many aspects of mammalian physiology. Mammals
are composed of distinct cell type identities and tissue environments, but the influence of …

Novel insights from a multiomics dissection of the Hayflick limit

M Chan, H Yuan, I Soifer, TM Maile, RY Wang… - Elife, 2022 - elifesciences.org
The process wherein dividing cells exhaust proliferative capacity and enter into replicative
senescence has become a prominent model for cellular aging in vitro. Despite decades of …

Gametogenesis: exploring an endogenous rejuvenation program to understand cellular aging and quality control

TL Sing, GA Brar, E Ünal - Annual review of genetics, 2022 - annualreviews.org
Gametogenesis is a conserved developmental program whereby a diploid progenitor cell
differentiates into haploid gametes, the precursors for sexually reproducing organisms. In …

rDNA array length is a major determinant of replicative lifespan in budding yeast

M Hotz, NH Thayer, DG Hendrickson… - Proceedings of the …, 2022 - National Acad Sciences
The complex processes and interactions that regulate aging and determine lifespan are not
fully defined for any organism. Here, taking advantage of recent technological advances in …

A genome‐scale yeast library with inducible expression of individual genes

Y Arita, G Kim, Z Li, H Friesen, G Turco… - Molecular Systems …, 2021 - embopress.org
The ability to switch a gene from off to on and monitor dynamic changes provides a powerful
approach for probing gene function and elucidating causal regulatory relationships. Here …

Cell cycle-linked vacuolar pH dynamics regulate amino acid homeostasis and cell growth

V Okreglak, R Ling, M Ingaramo, NH Thayer… - Nature …, 2023 - nature.com
Amino acid homeostasis is critical for many cellular processes. It is well established that
amino acids are compartmentalized using pH gradients generated between organelles and …

Advances in quantitative biology methods for studying replicative aging in Saccharomyces cerevisiae

R O'Laughlin, M Jin, Y Li, L Pillus, LS Tsimring… - … medicine of aging, 2020 - Elsevier
Aging is a complex, yet pervasive phenomenon in biology. As human cells steadily succumb
to the deteriorating effects of aging, so too comes a host of age-related ailments such as …

Age-dependent aggregation of ribosomal RNA-binding proteins links deterioration in chromatin stability with challenges to proteostasis

J Paxman, Z Zhou, R O'Laughlin, Y Liu, Y Li, W Tian… - Elife, 2022 - elifesciences.org
Chromatin instability and protein homeostasis (proteostasis) stress are two well-established
hallmarks of aging, which have been considered largely independent of each other. Using …

Tom70-based transcriptional regulation of mitochondrial biogenesis and aging

Q Liu, CE Chang, AC Wooldredge, B Fong… - Elife, 2022 - elifesciences.org
Mitochondrial biogenesis has two major steps: the transcriptional activation of nuclear
genome-encoded mitochondrial proteins and the import of nascent mitochondrial proteins …

Senescence in yeast is associated with amplified linear fragments of chromosome XII rather than ribosomal DNA circle accumulation

A Zylstra, H Hadj-Moussa, D Horkai, AJ Whale… - PLoS …, 2023 - journals.plos.org
The massive accumulation of extrachromosomal ribosomal DNA circles (ERCs) in yeast
mother cells has been long cited as the primary driver of replicative ageing. ERCs arise …