Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Multicellular spheroids and organoids are promising in vitro 3D models in personalized
medicine and drug screening. They replicate the structural and functional characteristics of …

Super-resolution imaging has rapidly emerged as an optical microscopy technique, offering
advantages of high optical resolution over the past two decades; achieving improved …

Fresnel incoherent correlation holography (FINCH) was a milestone in incoherent
holography. In this roadmap, two pathways, namely the development of FINCH and …

Fluorescence imaging techniques play a pivotal role in our understanding of the nervous
system. The emergence of various super-resolution microscopy methods and specialized …

Nanometric probes based on surface-enhanced Raman scattering (SERS) are promising
candidates for all-optical environmental, biological and technological sensing applications …

Distance measurements are commonly performed by phase detection based on a lock-in
strategy. Super-resolution fluorescence microscopy is still striving to perform axial …

Single-molecule localization microscopy (SMLM), while well established for cultured cells, is
not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique …

NMDA receptors (NMDARs) play key roles in the use-dependent adaptation of glutamatergic
synapses underpinning memory formation. In the forebrain, these plastic processes involve …

Common cameras are only sensitive to the intensity of light, discarding an essential feature
of a light wave: its phase profile or, equivalently, its wavefront profile. This Review focuses …