Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

A compendium of single extracellular vesicle flow cytometry

JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Identification of storage conditions stabilizing extracellular vesicles preparations

A Görgens, G Corso, DW Hagey… - Journal of …, 2022 - Wiley Online Library
Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological
processes and hold great potential for therapeutic and diagnostic use. Despite significant …

Updating MISEV: Evolving the minimal requirements for studies of extracellular vesicles

KW Witwer, DCI Goberdhan, L O'Driscoll… - Journal of …, 2021 - Wiley Online Library
The minimal information for studies of extracellular vesicles (EVs, MISEV) is a field‐
consensus rigour initiative of the International Society for Extracellular Vesicles (ISEV). The …

Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

U Erdbrügger, CJ Blijdorp, IV Bijnsdorp… - Journal of …, 2021 - Wiley Online Library
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of
extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last …

The impact of storage on extracellular vesicles: A systematic study

S Gelibter, G Marostica, A Mandelli… - Journal of …, 2022 - Wiley Online Library
Mounting evidence suggests that storage has an impact on extracellular vesicles (EVs)
properties. While− 80° C storage is a widespread approach, some authors proposed …

Graphene sensor arrays for rapid and accurate detection of pancreatic cancer exosomes in patients' blood plasma samples

T Yin, L Xu, B Gil, N Merali, MS Sokolikova… - ACS …, 2023 - ACS Publications
Biosensors based on graphene field effect transistors (GFETs) have the potential to enable
the development of point-of-care diagnostic tools for early stage disease detection …

MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research

F Lucien, D Gustafson, M Lenassi, B Li… - Journal of …, 2023 - Wiley Online Library
Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The
composition of a blood sample and its derivatives (ie, plasma and serum) are not only donor …

A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties

K Dooley, RE McConnell, K Xu, ND Lewis, S Haupt… - Molecular Therapy, 2021 - cell.com
Extracellular vesicles (EVs) are an important intercellular communication system facilitating
the transfer of macromolecules between cells. Delivery of exogenous cargo tethered to the …